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Citation
Hirosawa-Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbrüggen, G., Jäckle, H. (2009). A novel stem loop control element-dependent UGA read-through system without translational selenocysteine incorporation in Drosophila.  FASEB J. 23(1): 107--113.
FlyBase ID
FBrf0207157
Publication Type
Research paper
Abstract

Translational read-through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis-acting stem loop control element, termed SECIS, which is located in the 3'-untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS-directed UGA read-through process, we identified an evolutionary conserved GTPase-activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS-dependent UGA read-through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS-dependent translational read-through system that does not involve selenocysteine incorporation.

PubMed ID
PubMed Central ID
PMC2626620 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    FASEB J.
    Title
    FASEB Journal (Federation of American Societies for Experimental Biology)
    Publication Year
    1987-
    ISBN/ISSN
    0892-6638
    Data From Reference
    Alleles (6)
    Genes (7)
    Physical Interactions (3)
    Natural transposons (1)
    Insertions (1)
    Transgenic Constructs (4)