Reference Report

Reference
Citation	Martins, T., Maia, A.F., Steffensen, S., Sunkel, C.E. (2009). Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization. EMBO J. 28(3): 234--247. (Export to RIS)
FlyBase ID	FBrf0207175
Publication Type	Research paper
PubMed ID	19131964
PubMed Abstract	Sgt1 was described previously in yeast and humans to be a Hsp90 co-chaperone and required for kinetochore assembly. We have identified a mutant allele of Sgt1 in Drosophila and characterized its function. Mutations in sgt1 do not affect overall kinetochore assembly or spindle assembly checkpoint. sgt1 mutant cells enter less frequently into mitosis and arrest in a prometaphase-like state. Mutations in sgt1 severely compromise the organization and function of the mitotic apparatus. In these cells, centrioles replicate but centrosomes fail to mature, and pericentriolar material components do not localize normally resulting in highly abnormal spindles. Interestingly, a similar phenotype was described previously in Hsp90 mutant cells and correlated with a decrease in Polo protein levels. In sgt1 mutant neuroblasts, we also observe a decrease in overall levels of Polo. Overexpression of the kinase results in a substantial rescue of the centrosome defects; most cells form normal bipolar spindles and progress through mitosis normally. Taken together, these findings suggest that Sgt1 is involved in the stabilization of Polo allowing normal centrosome maturation, entry and progression though mitosis.
DOI	10.1038/emboj.2008.283
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (1)
	Df(3R)CA3
Alleles (8)
	Bub3¹ cid^{T:Disc\RFP-mRFP} cp309^{PACT.Ubi-p63E.T:Avic\GFP} polo^{Scer\UAS.P\T.cMa} Scer\GAL4^da.G32 Sgt1^c01268 Sgt1^c01428 αTub84B^{Ubi-p63E.T:Avic\GFP-S65C}
Constructs (5)
	P{GAL4-da.G32} P{gcid-mRFP-cid} P{UASp-polo.M} P{Ubi-p63E-GFP-cp309.PACT} P{Ubi-p63E-GFP-α-Tub84B}
Genes (13)
	Bub3 BubR1 Cenp-C cid cmet cnn cp309 CycB polo Scer\GAL4 Sgt1 αTub84B γTub23C
Insertions (2)
	PBac{PB}Sgt1^c01268 PBac{PB}Sgt1^c01428