Reference Report

Reference
Citation	Rivas, M.L., Cobreros, L., Zeidler, M.P., Castelli-Gair Hombria, J. (2008). Plasticity of Drosophila Stat DNA binding shows an evolutionary basis for Stat transcription factor preferences. EMBO Rep. 9(11): 1114--1120. (Export to RIS)
FlyBase ID	FBrf0207234
Publication Type	Research paper
PubMed ID	18802449
PubMed Abstract	In vertebrates, seven signal transducer and activator of transcription (STAT) proteins bind to palindromic sites separated by spacers of two or three nucleotides (STAT1), four nucleotides (STAT6) or three nucleotides (STAT2 to STAT5a/b). This diversity of binding sites provides specificity to counter semiredundancy and was thought to be a recent evolutionary acquisition. Here, we examine the natural DNA-binding sites of the single Drosophila Stat and show that this is not the case. Rather, Drosophila Stat92E is able to bind to and activate target gene expression through both 3n and 4n spaced sites. Our experiments indicate that Stat92E has a higher binding affinity for 3n sites than for 4n sites and suggest that the levels of target gene expression can be modulated by insertion and/or deletion of single bases. Our results indicate that the ancestral STAT protein had the capacity to bind to 3n and 4n sites and that specific STAT binding preferences evolved with the radiation of the vertebrate STAT family.
DOI	10.1038/embor.2008.170
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO Rep.
Title	EMBO Reports
Publication Year	2000-
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Data from Reference
Aberrations (1)
	Df(1)os1A2
Alleles (18)
	Ecol\lacZ^crb.3n-- Ecol\lacZ^crb.-4n4n Ecol\lacZ^crb.43.2 Ecol\lacZ^crb.STAT.mut Ecol\lacZ^dome.SB Ecol\lacZ^dome.SL Ecol\lacZ^{dome.SLF2.MUT3rd} Ecol\lacZ^{dome.SLF2.MUT4th} Ecol\lacZ^{dome.SLF2.MUT} Ecol\lacZ^dome.SLF2 Ecol\lacZ^{dome.SLF4.2n3n} Ecol\lacZ^{dome.SLF4.4n3n} Ecol\lacZ^{dome.SLF4.MUT} Ecol\lacZ^dome.SLF4 Ecol\lacZ^MESO.dome.hs Mef2^Scer\UAS.cBa os^Scer\UAS.cZa Scer\GAL4^69B
Constructs (15)
	P{crb-lacZ.3n--} P{crb-lacZ.-4n4n} P{crb-lacZ.43.2} P{crb-lacZ.STAT.mut} P{dome-lacZ.MESO} P{dome-lacZ.SB} P{dome-lacZ.SL} P{dome-lacZ.SLF2.MUT3rd} P{dome-lacZ.SLF2.MUT4th} P{dome-lacZ.SLF2.MUT} P{dome-lacZ.SLF2} P{dome-lacZ.SLF4.2n3n} P{dome-lacZ.SLF4.4n3n} P{dome-lacZ.SLF4.MUT} P{dome-lacZ.SLF4}
Genes (13)
	bap bin crb dome Ecol\lacZ hop Mef2 os Scer\GAL4 Stat92E tin upd2 upd3
Insertions (1)
	P{GawB}69B
Natural transposons (1)
	P-element