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Citation
Bosveld, F., Rana, A., Lemstra, W., Kampinga, H.H., Sibon, O.C. (2008). Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis.  BMC Res. Notes 1(): 75.
FlyBase ID
FBrf0207260
Publication Type
Research paper
Abstract

Coenzyme A (CoA) is an essential metabolite, synthesized from vitamin B5 by the subsequent action of five enzymes: PANK, PPCS, PPCDC, PPAT and DPCK. Mutations in Drosophila dPPCS disrupt female fecundity and in this study we analyzed the female sterile phenotype of dPPCS mutants in detail.We demonstrate that dPPCS is required for various processes that occur during oogenesis including chorion patterning. Our analysis demonstrates that a mutation in dPPCS disrupts the organization of the somatic and germ line cells, affects F-actin organization and results in abnormal PtdIns(4,5)P2 localization. Improper cell organization coincides with aberrant localization of the membrane molecules Gurken (Grk) and Notch, whose activities are required for specification of the follicle cells that pattern the eggshell. Mutations in dPPCS also induce alterations in scutellar patterning and cause wing vein abnormalities. Interestingly, mutations in dPANK and dPPAT-DPCK result in similar patterning defects.Together, our results demonstrate that de novo CoA biosynthesis is required for proper tissue morphogenesis.

PubMed ID
PubMed Central ID
PMC2542404 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Res. Notes
    Title
    BMC research notes
    ISBN/ISSN
    1756-0500
    Data From Reference
    Alleles (5)
    Genes (11)
    Transgenic Constructs (3)