Reference Report

Reference
Citation	Lin, M.D., Jiao, X., Grima, D., Newbury, S.F., Kiledjian, M., Chou, T.B. (2008). Drosophila processing bodies in oogenesis. Dev. Biol. 322(2): 276--288. (Export to RIS)
FlyBase ID	FBrf0207283
Publication Type	Research paper
PubMed ID	18708044
PubMed Abstract	Processing bodies (P-bodies) have emerged as important subcellular structures that are involved in mRNA metabolism. To date, a detailed description of P-bodies in Drosophila oogenesis is lacking. To this end, we first demonstrate that Drosophila decapping protein 2 (dDcp2) contains intrinsic decapping activity and its enzymatic activity was not detectably enhanced by Drosophila decapping protein 1 (dDcp1). dDcp1-containing bodies in the nurse cell cytoplasm can associate with the 5' to 3' exoribonuclease, Pacman in addition to dDcp2 and Me31B. The size and number of dDcp1 bodies are dynamic and dramatically increased in dDcp2 and pacman mutant backgrounds supporting the conclusion that dDcp1 bodies in nurse cell cytoplasm are Drosophila P-bodies. In stage 2-6 oocytes, dDcp1 bodies appear to be distinct from previously characterized P-bodies since they are insensitive to cycloheximide and RNase A treatments. Curiously, dDcp2 and Pacman do not colocalize with dDcp1 at the posterior end of the oocyte in stage 9-10 oocytes. This suggests that dDcp1 bodies are in a developmentally distinct state separate from the 5' end mRNA degradation enzymes at later stages in the oocyte. Interestingly, re-formation of maternally expressed dDcp1 with dDcp2 and Pacman was observed in early embryogenesis. With respect to developmental switching, the maternal dDcp1 is proposed to serve as a marker for the re-formation of P-bodies in early embryos. This also suggests that a regulated conversion occurs between maternal RNA granules and P-bodies from oogenesis to embryogenesis.
DOI	10.1016/j.ydbio.2008.07.033
Related Publication(s)
Supplementary material	Drosophila processing bodies in oogenesis. Supplementary data. [FBrf0214680]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(1)JA27
Alleles (12)
	Dcp1^442P Dcp1^{T:Avic\GFP-EYFP} Dcp1^tT2.R57A Dcp1^{αTub67C.T:Avic\GFP} Dcp2^de21 me31B^{vas.T:Avic\GFP-EGFP} osk^T:MS2\MCP.BS pcm³ pcm⁵ Scer\GAL4^{nos.UTR.T:Hsim\VP16} stau^{αTub67C.T:Avic\GFP-m6} twin^{Scer\UAS.P\T.T:Ivir\HA1}
Constructs (8)
	P{Dcp1.T2.R57A} P{GAL4::VP16-nos.UTR} P{osk-(ms2)6} P{UASp-twin.HA} P{vas-GFP-me31B.N} P{YFP-Dcp1.L} P{αTub67C-Dcp1.GFP} P{αTub67C-GFP-stau}
Genes (10)
	Dcp1 Dcp2 Dis3 me31B osk pcm Scer\GAL4 stau T:Avic\GFP twin
Insertions (1)
	P{EP}EP1526
Natural transposons (1)
	P-element