Reference Report

Reference
Citation	Morey, M., Yee, S.K., Herman, T., Nern, A., Blanco, E., Zipursky, S.L. (2008). Coordinate control of synaptic-layer specificity and rhodopsins in photoreceptor neurons. Nature 456(7223): 795--799. (Export to RIS)
FlyBase ID	FBrf0207285
Publication Type	Research paper
PubMed ID	18978774
PubMed Abstract	How neurons make specific synaptic connections is a central question in neurobiology. The targeting of the Drosophila R7 and R8 photoreceptor axons to different synaptic layers in the brain provides a model with which to explore the genetic programs regulating target specificity. In principle this can be accomplished by cell-type-specific molecules mediating the recognition between synaptic partners. Alternatively, specificity could also be achieved through cell-type-specific repression of particular targeting molecules. Here we show that a key step in the targeting of the R7 neuron is the active repression of the R8 targeting program. Repression is dependent on NF-YC, a subunit of the NF-Y (nuclear factor Y) transcription factor. In the absence of NF-YC, R7 axons terminate in the same layer as R8 axons. Genetic experiments indicate that this is due solely to the derepression of the R8-specific transcription factor Senseless (Sens) late in R7 differentiation. Sens is sufficient to control R8 targeting specificity and we demonstrate that Sens directly binds to an evolutionarily conserved DNA sequence upstream of the start of transcription of an R8-specific cell-surface protein, Capricious (Caps) that regulates R8 target specificity. We show that R7 targeting requires the R7-specific transcription factor Prospero (Pros) in parallel to repression of the R8 targeting pathway by NF-YC. Previous studies demonstrated that Sens and Pros directly regulate the expression of specific rhodopsins in R8 and R7. We propose that the use of the same transcription factors to promote the cell-type-specific expression of sensory receptors and cell-surface proteins regulating synaptic target specificity provides a simple and general mechanism for ensuring that transmission of sensory information is processed by the appropriate specialized neural circuits.
DOI	10.1038/nature07419
Related Publication(s)
Supplementary material	Supplementary Information. [FBrf0209766]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (13)
	caps^C18fs Ecol\lacZ^caps-E2-3-27 Ecol\lacZ^klg-H214 Ecol\lacZ^rho-BB02 Nf-YC⁴⁰ Nf-YC^Scer\UAS.cMa pros^17.17 Scer\GAL4^sca-109-68 Scer\GAL4^sev.PM181 Scer\GAL4^tub.PU Scer\GAL4^unspecified sens^E1 sens^Scer\UAS.cNa
Constructs (3)
	P{sev-GAL4.PM181} P{UAS-Nf-YC.M} P{UAS-sens.N}
Genes (15)
	ac ato boss caps chp dpn Ecol\lacZ klg Nf-YC pros run sca Scer\GAL4 sens sev
Insertions (4)
	P{A92}rho^BB02 P{GawB}sca^109-68 P{HZ}klg^H214 P{lacW}caps^E2-3-27