Reference Report

Reference
Citation	Wiklund, M.L., Steinert, S., Junell, A., Hultmark, D., Stöven, S. (2009). The N-terminal half of the Drosophila Rel/NF-kappaB factor Relish, REL-68, constitutively activates transcription of specific Relish target genes. Dev. Comp. Immunol. 33(5): 690--696. (Export to RIS)
FlyBase ID	FBrf0207341
Publication Type	Research paper
PubMed ID	19135474
PubMed Abstract	The Rel/NF-kappaB transcription factor Relish is a major regulator of the antimicrobial response in Drosophila. Upon immune challenge, Relish is cleaved to generate two fragments, the DNA-binding transcription factor REL-68 and the IkappaB-like REL-49. Using transgenic fly strains we show here that overexpression of REL-68 separately from REL-49 is sufficient to activate strong constitutive transcription of the Diptericin gene, but little constitutive or inducible transcription of Attacin and Cecropin, two other Relish target genes. Their transcription may therefore require additional modifications of Relish. However, phosphorylation of the conserved serine residue S431 is not involved in such modifications. This is unlike p65 and Dorsal, which are modulated by phosphorylation at their homologous site. In contrast to other IkappaB proteins, overexpression of REL-49 had no inhibitory effect on Relish-dependent transcription. Instead, we propose that the C-terminal IkappaB-like domain executes a scaffolding and recruiting function for full activation of Relish.
DOI	10.1016/j.dci.2008.12.002
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Comp. Immunol.
Title	Developmental and Comparative Immunology
Publication Year	1977-
ISBN/ISSN	0145-305X
Data from Reference
Alleles (6)
	Rel^{49.Scer\UAS.T:SV5\V5} Rel^{68.Scer\UAS.T:Zzzz\FLAG} Rel^{110.Scer\UAS.T:Hsap\MYC,T:Ivir\HA1} Rel^E20 Scer\GAL4^c729 Scer\GAL4^fat
Constructs (3)
	P{UAS-FLAG-Rel.68} P{UAS-myc-Rel-HA.110} P{UAS-Rel-V5.49}
Genes (11)
	AttA AttB CecA1 CecA2 Dpt Rel Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 T:SV5\V5 T:Zzzz\FLAG
Insertions (2)
	P{GAL4}fat P{GawB}c729
Natural transposons (1)
	P-element