Reference Report

Reference
Citation	Kappeler, M., Kranz, E., Woolcock, K., Georgiev, O., Schaffner, W. (2008). Drosophila bloom helicase maintains genome integrity by inhibiting recombination between divergent DNA sequences. Nucleic Acids Res. 36(21): 6907--6917. (Export to RIS)
FlyBase ID	FBrf0207350
Publication Type	Research paper
PubMed ID	18978019
PubMed Abstract	DNA double strand breaks (DSB) can be repaired either via a sequence independent joining of DNA ends or via homologous recombination. We established a detection system in Drosophila melanogaster to investigate the impact of sequence constraints on the usage of the homology based DSB repair via single strand annealing (SSA), which leads to recombination between direct repeats with concomitant loss of one repeat copy. First of all, we find the SSA frequency to be inversely proportional to the spacer length between the repeats, for spacers up to 2.4 kb in length. We further show that SSA between divergent repeats (homeologous SSA) is suppressed in cell cultures and in vivo in a sensitive manner, recognizing sequence divergences smaller than 0.5%. Finally, we demonstrate that the suppression of homeologous SSA depends on the Bloom helicase (Blm), encoded by the Drosophila gene mus309. Suppression of homeologous recombination is a novel function of Blm in ensuring genomic integrity, not described to date in mammalian systems. Unexpectedly, distinct from its function in Saccharomyces cerevisiae, the mismatch repair factor Msh2 encoded by spel1 does not suppress homeologous SSA in Drosophila.
DOI	10.1093/nar/gkn793
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nucleic Acids Res.
Title	Nucleic Acids Research
Publication Year	1974-
ISBN/ISSN	0305-1048
Data from Reference
Aberrations (2)
	Df(2L)b84h1 Df(2L)k08712-rv21
Alleles (12)
	Blm^D2 Blm^D3 Disc\RFP^3xP3.PB MtnB^{2x259gen.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{2xmutgen.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{cDNA-gen.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{gen.Scer\SceI.RS.MtnD.T:Avic\GFP-eGFP} MtnB^{gen-cDNA.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{mutcDNA.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{mutgen.Scer\SceI.RS.T:Avic\GFP-EGFP} MtnB^{T:Avic\GFP-EGFP} T:Avic\GFP^EGFP
Constructs (9)
	M{3xP3-RFP.attP} P{MtnBcDNA-cDNA^.EGFP} P{MtnBcDNA-gen.EGFP} P{MtnBcDNA-MtnDcDNA.EGFP} P{MtnBgen^-gen.EGFP} P{MtnBgen^-gen^.EGFP} P{MtnBgen-cDNA.EGFP} P{MtnBgen-EGFP} P{MtnBgen-gen.EGFP}
Genes (5)
	Blm Disc\RFP MtnB MtnD spel1
Natural transposons (1)
	P-element