Reference Report

Reference
Citation	Terriente, J., Perea, D., Suzanne, M., Díaz-Benjumea, F.J. (2008). The Drosophila gene zfh2 is required to establish proximal-distal domains in the wing disc. Dev. Biol. 320(1): 102--112. (Export to RIS)
FlyBase ID	FBrf0207390
Publication Type	Research paper
PubMed ID	18571155
PubMed Abstract	Three main events characterize the development of the proximal-distal axis of the Drosophila wing disc: first, generation of nested circular domains defined by different combinations of gene expression; second, activation of wingless (wg) gene expression in a ring of cells; and third, an increase of cell number in each domain in response to Wg. The mechanisms by which these domains of gene expression are established and maintained are unknown. We have analyzed the role of the gene zinc finger homeodomain 2 (zfh2). We report that in discs lacking zfh2 the limits of the expression domains of the genes tsh, nub, rn, dve and nab coincide, and expression of wg in the wing hinge, is lost. We show that zfh2 expression is delimited distally by Vg, Nub and Dpp signalling, and proximally by Tsh and Dpp. Distal repression of zfh2 permits activation of nab in the wing blade and wg in the wing hinge. We suggest that the proximal-most wing fate, the hinge, is specified first and that later repression of zfh2 permits specification of the distal-most fate, the wing blade. We propose that proximal-distal axis development is achieved by a combination of two strategies: on one hand a process involving proximal to distal specification, with the wing hinge specified first followed later by the distal wing blade; on the other hand, early specification of the proximal-distal domains by different combinations of gene expression. The results we present here indicate that Zfh2 plays a critical role in both processes.
DOI	10.1016/j.ydbio.2008.04.028
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(2R)01D01W-L186 Df(3R)rn20
Alleles (22)
	Avic\GFP^Scer\UAS.cUa dve^5.0.Scer\UAS Ecol\lacZ^rn-89 nab^SH143 nub¹ nub^R5 rn^{Scer\UAS.cSPa} Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^LP30 Scer\GAL4^NP1550 Scer\GAL4^nub-AC-62 Scer\GAL80^ts.αTub84B sgg^Scer\UAS.cBa tkv⁴ tkv^{Q199D.Scer\UAS.T:Ivir\HA1} tkv^Scer\UAS.cNa tsh^{dsRNA.Scer\UAS} tsh^Scer\UAS.cWa zfh2^{dsRNA.Scer\UAS} zfh2^MS209 zfh2^MS209R20
Constructs (11)
	P{GAL4-dpp.blk1} P{tubP-GAL80^ts} P{UAS-dve.5.0} P{UAS-GFP.U} P{UAS-rn.SP} P{UAS-sgg.B} P{UAS-tkv.Na} P{UAS-tkv^Q199D} P{UAS-tsh.dsRNA} P{UAS-tsh.W} P{UAS-zfh2.RNAi}
Genes (19)
	Avic\GFP dpp ds dve Ecol\lacZ elB fj nab noc nub rn Scer\GAL4 Scer\GAL80 sgg tkv tsh vg wg zfh2
Insertions (7)
	P{en2.4-GAL4}e16E P{GAL4}zfh2^MS209 P{GawB}dve^NP1550 P{GawB}LP30 P{GawB}nubbin-AC-62 P{lacW}nab^SH143 P{lArB}rn⁸⁹
Natural transposons (1)
	P-element