Home
Reference Report
| Reference | |||
|---|---|---|---|
| Citation | Christensen, S., Cook, K., Cook, K. (2009.2.28). Isolation and characterization of Df(3R)BSC744. (Export to RIS) | ||
| FlyBase ID | FBrf0207399 | ||
| Publication Type | Personal communication to FlyBase | ||
| PubMed ID | |||
| PubMed Abstract | |||
| Text of Personal Communication |
From: kercook@XXXXXXXXXXXXXXX
Subject: Isolation and characterization of Df(3R)BSC744 Date: 28 February 2009 17:35:13 GMT Isolation and characterization of Df(3R)BSC744 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC744 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG17917f06982 and P{XP}d03025. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG17917f06982/P{XP}d03025 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC744 from the segment of PBac{WH}CG17917f06982 to the left of its FRT site and the segment of P{XP}d03025 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d03025 to be Release 3 genomic coordinate 2231048 on chromosome arm 3R. This corresponds to 83E6 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}CG17917f06982 on the Release 5 map is 83E4. Consequently, the cytological breakpoints of Df(3R)BSC744 are predicted to be 83E4;83E6. Df(3R)BSC744 failed to complement Df(3R)BSC193 and Df(3R)WIN11. It was also lethal when combined with TM6B, Tb1, but the reason for this lethality is unclear. -- |
||
| DOI | |||
| Related Publication(s) | |||
Recent Updates
|
|||
| Description |
What does this section display?
This section contains items that were added to this record for each release.
It currently only tracks new links between this FlyBase report and other
FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
|
||
| Update Feed |
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your
feed reader.
|
||
| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
|
Associated Information
|
|||
| Comments | |||
| Associated Files | |||
|
Other Information
|
|||
| Secondary IDs | |||
| Language of Publication | English | ||
| Additional Languages of Abstract | |||
| Also Published As | |||
|
Parent Publication
|
|||
| Publication Type | |||
| Abbreviation | |||
| Title | |||
| ISBN/ISSN | |||
|
Data from Reference
|
|||
| Aberrations (3)
|
|||
| Constructs (1)
|
|||
| Insertions (3)
|
|||