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Christensen, S., Cook, K., Cook, K. (2009.2.28). Isolation and characterization of Df(3L)BSC753. 
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FBrf0207408
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From: kercook@XXXX
Subject: Isolation and characterization of Df(3L)BSC753
Date: February 28, 2009  12:36:18  PM GMT- 05:00 
To: flybase-cambridgeXXXX, sjchristXXXX, ruacookXXXX, kaufmanXXXX
Isolation and characterization of Df(3L)BSC753
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC753 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03517 and PBac{RB}e01116. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d03517/PBac{RB}e01116 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC753 from the segment of P{XP}d03517 to the left of its FRT site and the segment of PBac{RB}e01116 to the right of its FRT site. The cytological breakpoints of Df(3L)BSC753 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 68A4;68C9. Df(3L)BSC753 failed to complement l(3)01239j9B4 and Sodn1.
-- 
Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN  47405-7005
kercook@XXXX
812-856-1213 (office), 812-855-2577 (fax)
http://flystocks.bio.indiana.edu
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    English
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)