Isolation and characterization of Df(1)BSC754
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC754 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f07799 and P{XP}d04142. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f07799/P{XP}d04142; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC754 from the segment of PBac{WH}f07799 to the left of its FRT site and the segment of P{XP}d04142 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC754 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 9A4;9B4. It failed to complement Yp1ts1.