Isolation and characterization of Df(1)BSC755
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC755 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02181 and PBac{RB}CG17333e04318. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}d02181/PBac{RB}CG17333e04318; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC755 from the segment of P{XP}d02181 to the left of its FRT site and the segment of PBac{RB}CG17333e04318 to the right of its FRT site. The cytological breakpoints of Df(1)BSC755 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 9C4;9F5. It failed to complement ras2.