Reference Report

Reference
Citation	Bahn, J.H., Lee, G., Park, J.H. (2009). Comparative analysis of Pdf-mediated circadian behaviors between Drosophila melanogaster and D. virilis. Genetics 181(3): 965--975. (Export to RIS)
FlyBase ID	FBrf0207444
Publication Type	Research paper
PubMed ID	19153257
PubMed Abstract	A group of small ventrolateral neurons (s-LN(v)'s) are the principal pacemaker for circadian locomotor rhythmicity of Drosophila melanogaster, and the pigment-dispersing factor (Pdf) neuropeptide plays an essential role as a clock messenger within these neurons. In our comparative studies on Pdf-associated circadian rhythms, we found that daily locomotor activity patterns of D. virilis were significantly different from those of D. melanogaster. Activities of D. virilis adults were mainly restricted to the photophase under light:dark cycles and subsequently became arrhythmic or weakly rhythmic in constant conditions. Such activity patterns resemble those of Pdf(01) mutant of D. melanogaster. Intriguingly, endogenous D. virilis Pdf (DvPdf) expression was not detected in the s-LN(v)-like neurons in the adult brains, implying that the Pdf(01)-like behavioral phenotypes of D. virilis are attributed in part to the lack of DvPdf in the s-LN(v)-like neurons. Heterologous transgenic analysis showed that cis-regulatory elements of the DvPdf transgene are capable of directing their expression in all endogenous Pdf neurons including s-LN(v)'s, as well as in non-Pdf clock neurons (LN(d)'s and fifth s-LN(v)) in a D. melanogaster host. Together these findings suggest a significant difference in the regulatory mechanisms of Pdf transcription between the two species and such a difference is causally associated with species-specific establishment of daily locomotor activity patterns.
DOI	10.1534/genetics.108.099069
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (10)
	Clk^Jrk cyc⁰¹ cyc⁰² Dvir\Pdf^+t3.5 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pdf⁰¹ rpr^Scer\UAS.cZa Scer\GAL4^Dvir\Pdf.1.9 Scer\GAL4^npf.1 Scer\GAL4^P2.4.Pdf
Constructs (6)
	P{DvPdf.+3.5} P{DvPdf-GAL4.1.9} P{npf-GAL4.1} P{Pdf-GAL4.P2.4} P{UAS-mCD8::GFP.L} P{UAS-rpr.Z}
Genes (8)
	Clk cyc Dvir\Pdf Mmus\Cd8a Pdf rpr Scer\GAL4 tim
Natural transposons (1)
	P-element