Reference Report

Reference
Citation	Grusche, F.A., Hidalgo, C., Fletcher, G., Sung, H.H., Sahai, E., Thompson, B.J. (2009). Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]. BMC Dev. Biol. 9(): 14. (Export to RIS)
FlyBase ID	FBrf0207451
Publication Type	Research paper
PubMed ID	19228425
PubMed Abstract	How epithelial cells adopt their particular polarised forms is poorly understood. In a screen for genes regulating epithelial morphology in Drosophila, we identified sds22, a conserved gene previously characterised in yeast.In the columnar epithelia of imaginal discs or follicle cells, mutation of sds22 causes contraction of cells along their apical-basal axis, resulting in a more cuboidal morphology. In addition, the mutant cells can also display altered cell polarity, forming multiple layers in follicle cells and leaving the epithelium in imaginal discs. In yeast, sds22 encodes a PP1 phosphatase regulatory subunit. Consistent with this, we show that Drosophila Sds22 binds to all four Drosophila PP1s and shares an overlapping phenotype with PP1beta9c. We also show that two previously postulated PP1 targets, Spaghetti Squash and Moesin are hyper-phosphorylated in sds22 mutants. This function is shared by the human homologue of Sds22, PPP1R7.Sds22 is a conserved PP1 phosphatase regulatory subunit that controls cell shape and polarity.
DOI	10.1186/1471-213X-9-14
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Language of Publication	English
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Publication Type	Journal
Abbreviation	BMC Dev. Biol.
Title	BMC Developmental Biology
Publication Year	2002
ISBN/ISSN	1471-213X
Data from Reference
Alleles (9)
	BacA\p35^Scer\UAS.cHa Scer\GAL4^Act.PU Scer\GAL4^c204 Scer\GAL4^en-e16E Scer\GAL4^tub.PU sds22^GD11788 sds22^PB1173 sds22^{Scer\UAS.T:Avic\GFP-EGFP} T:Avic\GFP^EGFP
Constructs (5)
	P{GD11788} P{tub-GAL4.U} P{UAS-p35.H} P{UAS-sds22.EGFP} PBac{IRES-GFP.GT}
Genes (13)
	aPKC arm BacA\p35 dlg1 flw Moe Pp1-13C Pp1-87B Pp1α-96A Scer\GAL4 sds22 sqh α-Spec
Insertions (3)
	P{en2.4-GAL4}e16E P{GawB}c204 PBac{IRES-GFP.GT}sds22^PB1173
Natural transposons (1)
	P-element