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Nezis, I.P., Lamark, T., Velentzas, A.D., Rusten, T.E., Bjørkøy, G., Johansen, T., Papassideri, I.S., Stravopodis, D.J., Margaritis, L.H., Stenmark, H., Brech, A. (2009). Cell death during Drosophila melanogaster early oogenesis is mediated through autophagy.  Autophagy 5(3): 298--302.
FlyBase ID
FBrf0207457
Publication Type
Research paper
Abstract

Autophagy is a physiological and evolutionarily conserved process maintaining homeostatic functions, such as protein degradation and organelle turnover. Accumulating data provide evidence that autophagy also contributes to cell death under certain circumstances, but how this is achieved is not well known. Herein, we report that autophagy occurs during developmentally-induced cell death in the female germline, observed in the germarium and during middle developmental stages of oogenesis in Drosophila melanogaster. Degenerating germline cells exhibit caspase activation, chromatin condensation, DNA fragmentation and punctate staining of mCherry-DrAtg8a, a novel marker for monitoring autophagy in Drosophila. Genetic inhibition of autophagy, by removing atg1 or atg7 function, results in significant reduction of DNA fragmentation, suggesting that autophagy acts genetically upstream of DNA fragmentation in this tissue. This study provides new insights into the mechanisms that regulate cell death in vivo during development.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Autophagy
    Title
    Autophagy
    Publication Year
    2005-
    ISBN/ISSN
    1554-8627 1554-8635
    Data From Reference
    Alleles (5)
    Genes (5)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (3)