Reference Report

Reference
Citation	Akbar, M.A., Ray, S., Krämer, H. (2009). The SM protein Car/Vps33A regulates SNARE-mediated trafficking to lysosomes and lysosome-related organelles. Mol. Biol. Cell 20(6): 1705--1714. (Export to RIS)
FlyBase ID	FBrf0207605
Publication Type	Research paper
PubMed ID	19158398
PubMed Abstract	The SM proteins Vps33A and Vps33B are believed to act in membrane fusions in endosomal pathways, but their specific roles are controversial. In Drosophila, Vps33A is the product of the carnation (car) gene. We generated a null allele of car to test its requirement for trafficking to different organelles. Complete loss of car function is lethal during larval development. Eye-specific loss of Car causes late, light-independent degeneration of photoreceptor cells. Earlier in these cells, two distinct phenotypes were detected. In young adults, autophagosomes amassed indicating that their fusion with lysosomes requires Car. In eye discs, endocytosed receptors and ligands accumulate in Rab7-positive prelysosomal compartments. The requirement of Car for late endosome-to-lysosome fusion in imaginal discs is specific as early endosomes are unaffected. Furthermore, lysosomal delivery is not restored by expression of dVps33B. This specificity reflects the distinct pattern of binding to different Syntaxins in vitro: dVps33B predominantly binds the early endosomal Avl and Car to dSyntaxin16. Consistent with a role in Car-mediated fusion, dSyntaxin16 is not restricted to Golgi membranes but also present on lysosomes.
DOI	10.1091/mbc.E08-03-0282
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (4)
	Df(1)car^Δ33 Df(1)car^Δ141 Df(1)car^Δ163 Df(1)Exel6254
Alleles (25)
	car¹ car^+t4.4 car^G0447 car^Scer\UAS.cAa car^Δ33 car^Δ141 car^Δ146 car^Δ163 dor⁸ Grip84^t4.4 Grip84^Δ33 Grip84^Δ141 Grip84^Δ163 Hsap\LAMP1^{Scer\UAS.T:Avic\GFP-EGFP} Hsap\LAMP1^{tub.T:Avic\GFP-EGFP} Rab5^{αTub84B.T:Avic\GFP-ECFP} Rab7^{αTub84B.T:Avic\GFP-EYFP} Rab11^{αTub84B.T:Avic\GFP-EYFP} Scer\GAL4^da.G32 Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^tub.PU T:Avic\GFP^EGFP Vps33B^{Scer\UAS.T:Hsap\MYC} w^+mC
Constructs (12)
	P{car^+t4.4} P{GAL4-da.G32} P{GAL4-ey.H} P{GAL4-ninaE.GMR} P{tub-GAL4.U} P{tub-GFP-LAMP1} P{UAS-car.A} P{UAS-GFP-LAMP} P{UAS-Vps33B.Myc} P{αTub84B-Rab5.CFP} P{αTub84B-Rab7.YFP} P{αTub84B-Rab11.YFP}
Genes (27)
	boss car Dl dor Gp150 Grip84 hk Hrs Hsap\LAMP1 lva N os Rab5 Rab7 Rab11 Scer\GAL4 sw Syx7 Syx8 Syx13 Syx16 T:Hsap\MYC Vps16A Vps33B w wg γTub23C
Insertions (1)
	P{lacW}car^G0447
Natural transposons (1)
	P-element