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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2009.2.28). Isolation and characterization of Df(3L)BSC735. (Export to RIS) | ||
| FlyBase ID | FBrf0207617 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: kercook@XXXXXXXXXXXXXXX
Subject: Isolation and characterization of Df(3L)BSC735 Date: 28 February 2009 17:34:06 GMT To: flybase-cambridgeXXXXXXXXXXXXXXX, sjchristXXXXXXXXXXXXXXX, ruacookXXXXXXXXXXXXXXX, kaufmanXXXXXXXXXXXXXXX Isolation and characterization of Df(3L)BSC735 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC735 was isolated as a FLP recombinase-induced recombination event involving P{XP}Gad1d10811 and PBac{WH}CG18675f01891. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}Gad1d10811/PBac{WH}CG18675f01891 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC735 from the segment of P{XP}Gad1d10811 to the left of its FRT site and the segment of PBac{WH}CG18675f01891 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC735 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 64A5;64A9. Df(3L)BSC735 failed to complement RopG27, RfC40B6 and masxs76. -- Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXXXXXXXXXXXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (3)
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| Constructs (1)
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| Genes (3)
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| Insertions (3)
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