Reference Report

Reference
Citation	Widmann, T.J., Dahmann, C. (2009). Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. J. Cell Sci. 122(9): 1362--1373. (Export to RIS)
FlyBase ID	FBrf0207672
Publication Type	Research paper
PubMed ID	19366729
PubMed Abstract	Morphogenesis is largely driven by changes in the shape of individual cells. However, how cell shape is regulated in developing animals is not well understood. Here, we show that the onset of TGFbeta/Dpp signaling activity correlates with the transition from cuboidal to columnar cell shape in developing Drosophila melanogaster wing disc epithelia. Dpp signaling is necessary for maintaining this elongated columnar cell shape and overactivation of the Dpp signaling pathway results in precocious cell elongation. Moreover, we provide evidence that Dpp signaling controls the subcellular distribution of the activities of the small GTPase Rho1 and the regulatory light chain of non-muscle myosin II (MRLC). Alteration of Rho1 or MRLC activity has a profound effect on apical-basal cell length. Finally, we demonstrate that a decrease in Rho1 or MRLC activity rescues the shortening of cells with compromised Dpp signaling. Our results identify a cell-autonomous role for Dpp signaling in promoting and maintaining the elongated columnar shape of wing disc cells and suggest that Dpp signaling acts by regulating Rho1 and MRLC.
DOI	10.1242/jcs.044271
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(2L)flp147E
Alleles (27)
	Avic\GFP^vkg-G454 BacA\p35^Scer\UAS.cHa brk^XH Dad^Scer\UAS.cTa dia^GD9442 Ecol\lacZ^brk-X47 Kat60^Scer\UAS.cWa Klp10A^Scer\UAS.cWa Mad¹² Mad^B1 Mbs^{N300.Scer\UAS} Mbs^T666 Moe^{dsRNA.327-775.Scer\UAS} Pkn^{G58A.Scer\UAS.T:Avic\GFP-EGFP} Rho1^GD4726 Rho1^N19.Scer\UAS Rho1^V14.Scer\UAS RhoGEF2^Scer\UAS.cUa Scer\GAL4^ap-md544 Scer\GAL4^nub-AC-62 Scer\GAL80^ts.αTub84B sqh^{E20E21.Scer\UAS.T:Zzzz\FLAG} sqh^GD1695 tkv^{1ΔGSK.Scer\UAS} tkv⁴ tkv^{Q253D.Scer\UAS.cNb} tkv^Scer\UAS.cNa
Constructs (18)
	P{GD1695} P{GD4726} P{GD9442} P{tubP-GAL80^ts} P{UAS-Dad.T} P{UAS-kat60.W} P{UAS-Klp10A.W} P{UAS-Mbs.N300} P{UAS-Moe.IR.327-775} P{UAS-p35.H} P{UAS-Pkn.G58A.eGFP} P{UAS-Rho1.N19} P{UAS-Rho1.V14} P{UAS-RhoGEF2.U} P{UAS-sqh.E20E21.FLAG} P{UAS-tkv.1ΔGSK} P{UAS-tkv.Na} P{UAS-tkv.Q253D.Nb}
Genes (28)
	Avic\GFP BacA\p35 brk bsk crb Dad dia dlg1 Ecol\lacZ Fas3 Kat60 Klp10A Mad Mbs Moe mys p35 Pkn Rho1 RhoGEF2 salm Scer\GAL4 Scer\GAL80 shg sqh tkv twi wg
Insertions (4)
	P{GawB}ap^md544 P{GawB}nubbin-AC-62 P{PTT-un1}vkg^G454 P{PZ}X47
Natural transposons (1)
	P-element