|Citation||Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R., Yamaguchi, M. (2009). Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF. Exp. Cell Res. 315(8): 1403--1414. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||The Mes4 gene has been identified as one of the maternal Dorsal target genes in Drosophila. In the present study, we found a DNA replication-related element (DRE, 5'-TATCGATA) in the Mes4 promoter recognized by the DRE-binding factor (DREF). Luciferase transient expression assays in S2 cells using Mes4 promoter-luciferase fusion plasmids revealed that the DRE sequence is essential for Mes4 promoter activity. Requirement of DRE for Mes4 promoter activity was further confirmed by anti-beta-galactosidase antibody-staining of various tissues from transgenic flies carrying Mes4 promoter-lacZ fusion genes. Furthermore, wild type Mes4 promoter activity was decreased by 40% in DREF-depleted S2 cells. These results indicate that DREF positively regulates Mes4 gene expression. Band mobility shift analyses using Kc cell nuclear extracts further indicated that the DRE sequence in the Mes4 promoter is especially important for binding to DREF. Moreover, specific binding of DREF to the involved genomic region could be demonstrated by chromatin immunoprecipitation assays using anti-DREF antibodies. These results, taken together, indicate that the DRE/DREF system activates transcription of the Mes4 gene. In addition, knockdown of the Mes4 gene in wing imaginal discs using the GAL4-UAS system caused an atrophied wing phenotype, suggesting that Mes4 is required for wing morphogenesis.|
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|Language of Publication||English|
|Additional Languages of Abstract|
|Also Published As|
|Abbreviation||Exp. Cell Res.|
|Title||Experimental Cell Research|
|Data from Reference|
|Natural transposons (1)|
|Sequence features (1)|