Reference Report

Reference
Citation	Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R., Yamaguchi, M. (2009). Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF. Exp. Cell Res. 315(8): 1403--1414. (Export to RIS)
FlyBase ID	FBrf0207714
Publication Type	Research paper
PubMed ID	19150446
PubMed Abstract	The Mes4 gene has been identified as one of the maternal Dorsal target genes in Drosophila. In the present study, we found a DNA replication-related element (DRE, 5'-TATCGATA) in the Mes4 promoter recognized by the DRE-binding factor (DREF). Luciferase transient expression assays in S2 cells using Mes4 promoter-luciferase fusion plasmids revealed that the DRE sequence is essential for Mes4 promoter activity. Requirement of DRE for Mes4 promoter activity was further confirmed by anti-beta-galactosidase antibody-staining of various tissues from transgenic flies carrying Mes4 promoter-lacZ fusion genes. Furthermore, wild type Mes4 promoter activity was decreased by 40% in DREF-depleted S2 cells. These results indicate that DREF positively regulates Mes4 gene expression. Band mobility shift analyses using Kc cell nuclear extracts further indicated that the DRE sequence in the Mes4 promoter is especially important for binding to DREF. Moreover, specific binding of DREF to the involved genomic region could be demonstrated by chromatin immunoprecipitation assays using anti-DREF antibodies. These results, taken together, indicate that the DRE/DREF system activates transcription of the Mes4 gene. In addition, knockdown of the Mes4 gene in wing imaginal discs using the GAL4-UAS system caused an atrophied wing phenotype, suggesting that Mes4 is required for wing morphogenesis.
DOI	10.1016/j.yexcr.2008.12.017
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Exp. Cell Res.
Title	Experimental Cell Research
Publication Year	1950-
ISBN/ISSN	0014-4827
Data from Reference
Alleles (11)
	Ecol\lacZ^{Mes4.-294DREmut} Ecol\lacZ^Mes4.-294wt Mes4^{dsRNA.Scer\UAS.WIZ} Scer\GAL4^Act5C.PU Scer\GAL4^ap-md544 Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^GMR.PS Scer\GAL4^tub.PU w^+mC
Constructs (5)
	P{GAL4-GMR.S} P{Mes4-lacZ.-294DREmut} P{Mes4-lacZ.-294wt} P{tub-GAL4.U} P{UAS-Mes4.IR}
Genes (10)
	Dref Ecol\lacZ His3 Mes4 Nf-YA Nf-YB Nf-YC qkr58E-3 Scer\GAL4 w
Insertions (13)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096 P{Mes4-lacZ.-294DREmut}8 P{Mes4-lacZ.-294DREmut}10 P{Mes4-lacZ.-294DREmut}53 P{Mes4-lacZ.-294wt}3 P{Mes4-lacZ.-294wt}20 P{UAS-Mes4.IR}7 P{UAS-Mes4.IR}18 P{UAS-Mes4.IR}22 P{UAS-Mes4.IR}43-1 P{UAS-Mes4.IR}43-5 P{UAS-Mes4.IR}56
Natural transposons (1)
	P-element
Sequence features (1)
	Mes4_promoter