Reference Report

Reference
Citation	Choi, C.M., Vilain, S., Langen, M., Van Kelst, S., De Geest, N., Yan, J., Verstreken, P., Hassan, B.A. (2009). Conditional mutagenesis in Drosophila. Science 324(5923): 54. (Export to RIS)
FlyBase ID	FBrf0207727
Publication Type	Research paper
PubMed ID	19342580
PubMed Abstract	Most genes function at multiple stages of metazoan development, in dividing and nondividing cells. Generating mouse conditional knock-outs (cKO), where a gene can be eliminated in a temporally and spatially controlled manner, is a valuable technique because it allows study of gene function at any stage of life. In contrast and despite the development of many other powerful genetic tools, cKO has thus far been lacking in Drosophila. We combined several recent molecular and genetic technical advances in an approach termed integrase-mediated approach for gene knock-out (IMAGO). IMAGO allows the replacement of any genomic sequence, such as a gene, with another desired sequence, including cKO alleles that can be used to create positively marked mutant cells. IMAGO should also be applicable to other genetic model organisms.
DOI	10.1126/science.1168275
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Science
Title	Science
Publication Year	1895-
ISBN/ISSN	0036-8075
Data from Reference
Alleles (10)
	ato^ato.attR ato^FAFG4 ato^G4 ato^{Scer\GAL4.attR} ato^w.attR ato^{w.Scer\SceI.RS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\FLP1^ey.PN Scer\GAL4^ato.attR Scer\GAL4^ato.G4
Constructs (3)
	P{ey-FLP.N} P{FRT(attP(w⁺).ato^{Scer\SceI.RS.cWa})} P{UAS-mCD8::GFP.L}
Genes (9)
	ato Avic\GFP elav Mmus\Cd8a phiC31\attP phiC31\int Scer\FLP1 Scer\GAL4 sens
Natural transposons (1)
	P-element