Reference Report

Reference
Citation	Jang, A.C., Chang, Y.C., Bai, J., Montell, D. (2009). Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt. Nat. Cell Biol. 11(5): 569--579. (Export to RIS)
FlyBase ID	FBrf0207765
Publication Type	Research paper
PubMed ID	19350016
PubMed Abstract	During development, elaborate patterns of cell differentiation and movement must occur in the correct locations and at the proper times. Developmental timing has been studied less than spatial pattern formation, and the mechanisms integrating the two are poorly understood. Border-cell migration in the Drosophila ovary occurs specifically at stage 9. Timing of the migration is regulated by the steroid hormone ecdysone, whereas spatial patterning of the migratory population requires localized activity of the JAK-STAT pathway. Ecdysone signalling is patterned spatially as well as temporally, although the mechanisms are not well understood. In stage 9 egg chambers, ecdysone signalling is highest in anterior follicle cells including the border cells. We identify the gene abrupt as a repressor of ecdysone signalling and border-cell migration. Abrupt protein is normally lost from border-cell nuclei during stage 9, in response to JAK-STAT activity. This contributes to the spatial pattern of the ecdysone response. Abrupt attenuates ecdysone signalling by means of a direct interaction with the basic helix-loop-helix (bHLH) domain of the P160 ecdysone receptor coactivator Taiman (Tai). Taken together, these findings provide a molecular mechanism by which spatial and temporal cues are integrated.
DOI	10.1038/ncb1863
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Alleles (36)
	ab^1D ab²⁶ ab^clu-1 ab^EY09709 ab^k02807 ab^NP1107 ab^Scer\UAS.cUa Ecol\lacZ^Adh.EcRE Ecol\lacZ^slbo-01310 EcR^A.Scer\UAS EcR^B1.Scer\UAS EcR^{B1-ΔC655.F645A.Scer\UAS} EcR^{B1-ΔC655.W650A.Scer\UAS} EcR^B2.Scer\UAS EcR^{hs.T:Scer\GAL4} Egfr^DN.Scer\UAS hop^Scer\UAS.cHa hop^Tum.Scer\UAS Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pvr^DN.Scer\UAS Rac1^N17.Scer\UAS Scer\GAL4^c306 Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^slbo.2.6 Stat92E^EP3391 Stat92E^F tai^61G1 tai^{bHLH.Scer\UAS.T:Zzzz\FLAG,T:Uuuu\nls6} tai^{BP.Scer\UAS.T:Zzzz\FLAG,T:Uuuu\nls6} tai^{LXXLL.Scer\UAS.T:Avic\GFP,T:Uuuu\nls6} tai^{PAS.Scer\UAS.T:Zzzz\FLAG,T:Uuuu\nls6} tai^Scer\UAS.cBa tai^ΔB.Scer\UAS tai^ΔC.Scer\UAS tai^ΔP.Scer\UAS usp^{hs.T:Scer\GAL4}
Constructs (22)
	P{AyGAL4} P{EcRE.lacZ} P{GAL4-slbo.2.6} P{hs-GAL4-EcR.LBD} P{hs-GAL4-usp.LBD} P{UAS-ab.U} P{UAS-EcR.A} P{UAS-EcR.B1} P{UAS-EcR.B1-ΔC655.F645A} P{UAS-EcR.B1-ΔC655.W650A} P{UAS-EcR.B2} P{UAS-hop.H} P{UAS-hop.Tum} P{UAS-mCD8::GFP.L} P{UAS-tai.B} P{UAS-tai.Flag-nls.bHLH} P{UAS-tai.Flag-nls.BP} P{UAS-tai.Flag-nls.PAS} P{UAS-tai.nls.LXXLL-GFP} P{UAS-tai.ΔB} P{UAS-tai.ΔC} P{UAS-tai.ΔP}
Genes (18)
	ab arm Ecol\lacZ EcR Egfr hop Mmus\Cd8a Pvr Rac1 Scer\GAL4 slbo sn Stat92E T:Avic\GFP T:Uuuu\nls6 T:Zzzz\FLAG tai usp
Insertions (4)
	P{EPgy2}ab^EY09709 P{GawB}ab^NP1107 P{GawB}c306 P{PZ}slbo⁰¹³¹⁰
Natural transposons (1)
	P-element