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Furukawa, K., Ishida, K., Tsunoyama, T.A., Toda, S., Osoda, S., Horigome, T., Fisher, P.A., Sugiyama, S. (2009). A-type and B-type lamins initiate layer assembly at distinct areas of the nuclear envelope in living cells.  Exp. Cell Res. 315(7): 1181--1189.
FlyBase ID
FBrf0207782
Publication Type
Research paper
Abstract
To investigate nuclear lamina re-assembly in vivo, Drosophila A-type and B-type lamins were artificially expressed in Drosophila lamin Dm(0)null mutant brain cells. Both exogenous lamin C (A-type) and Dm(0) (B-type) formed sub-layers at the nuclear periphery, and efficiently reverted the abnormal clustering of the NPC. Lamin C initially appeared where NPCs were clustered, and subsequently extended along the nuclear periphery accompanied by the recovery of the regular distribution of NPCs. In contrast, lamin Dm(0) did not show association with the clustered NPCs during lamina formation and NPC spacing recovered only after completion of a closed lamin Dm(0) layer. Further, when lamin Dm(0) and C were both expressed, they did not co-polymerize, initiating layer formation in separate regions. Thus, A and B-type lamins reveal differing properties during lamina assembly, with A-type having the primary role in organizing NPC distribution. This previously unknown complexity in the assembly of the nuclear lamina could be the basis for intricate nuclear envelope functions.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Exp. Cell Res.
    Title
    Experimental Cell Research
    Publication Year
    1950-
    ISBN/ISSN
    0014-4827
    Data From Reference
    Alleles (6)
    Genes (4)
    Transgenic Constructs (5)