Reference Report

Reference
Citation	Raghu, P., Coessens, E., Manifava, M., Georgiev, P., Pettitt, T., Wood, E., Garcia-Murillas, I., Okkenhaug, H., Trivedi, D., Zhang, Q., Razzaq, A., Zaid, O., Wakelam, M., O'Kane, C.J., Ktistakis, N. (2009). Rhabdomere biogenesis in Drosophila photoreceptors is acutely sensitive to phosphatidic acid levels. J. Cell Biol. 185(1): 129--145. (Export to RIS)
FlyBase ID	FBrf0207819
Publication Type	Research paper
PubMed ID	19349583
PubMed Abstract	Phosphatidic acid (PA) is postulated to have both structural and signaling functions during membrane dynamics in animal cells. In this study, we show that before a critical time period during rhabdomere biogenesis in Drosophila melanogaster photoreceptors, elevated levels of PA disrupt membrane transport to the apical domain. Lipidomic analysis shows that this effect is associated with an increase in the abundance of a single, relatively minor molecular species of PA. These transport defects are dependent on the activation state of Arf1. Transport defects via PA generated by phospholipase D require the activity of type I phosphatidylinositol (PI) 4 phosphate 5 kinase, are phenocopied by knockdown of PI 4 kinase, and are associated with normal endoplasmic reticulum to Golgi transport. We propose that PA levels are critical for apical membrane transport events required for rhabdomere biogenesis.
DOI	10.1083/jcb.200807027
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (20)
	AP-2α^{dsRNA.Scer\UAS.cUa} Arf51F⁴ ArfGAP1^Scer\UAS.cRa CdsA¹ CG11426^{Scer\UAS.T:Avic\GFP} garz^EP2028 Pi4KIIα^GD9857 Pld^{K1086R.Scer\UAS} Pld^Scer\UAS.cRa rdgA^Scer\UAS.cRa Scer\GAL4^GMR.PF Scer\GAL4^hs.PB Scer\GAL4^ninaE.PT shi¹ siz^P2.Scer\UAS sktl^KD.Scer\UAS sktl^Scer\UAS.cRa sktl^Δ1-1 sktl^Δ20 w^+mC
Constructs (11)
	P{GAL4-Hsp70.PB} P{GAL4-ninaE.GMR} P{GD9857} P{rh1-GAL4} P{UAS-Gap69C.R} P{UAS-Pld.K1086R} P{UAS-Pld.R} P{UAS-rgdA.R} P{UAS-sktl.KD} P{UAS-sktl.R} P{UAS-α-Adaptin.RNAi.U}
Genes (18)
	AP-2α Arf51F ArfGAP1 CdsA CG11426 garz ninaE norpA PI4KIIIalpha Pi4KIIα Pld rdgA Scer\GAL4 shi siz sktl trp w
Natural transposons (1)
	P-element