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Christensen, S., Cook, K., Cook, K. (2009.5.6). Isolation and characterization of Df(2R)BSC785. 
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From: 	kercook@XXXX
	Subject: 	Isolation and characterization of Df(2R)BSC785
	Date: 	6 May 2009  00:41:13  BST
	To: 	flybase-cambridgeXXXX, sjchristXXXX, ruacook@XXXX
Isolation and characterization of Df(2R)BSC785
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC785 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG30185d11316 and PBac{RB}Pi3K59Fe03961. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG30185d11316/PBac{RB}Pi3K59Fe03961 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC785 from the segment of P{XP}CG30185d11316 to the left of its FRT site and the segment of PBac{RB}Pi3K59Fe03961 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(2R)BSC785 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:19431599 ;19447811 and the cytological breakpoints predicted from these coordinates are 59E3;59E4. Df(2R)BSC785 failed to complement wmdKG07581 for a visible wing phenotype and pita02132 for lethality.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           Indiana University              1001 E. Third St.               Bloomington, IN  47405-7005   
812-856-1213 (office), 812-855-2577 (fax)
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)