Isolation and characterization of Df(3R)BSC791
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC791 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG31077f01593 and P{XP}d06753. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG31077f01593/P{XP}d06753 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC791 from the segment of PBac{WH}CG31077f01593 to the left of its FRT site and the segment of P{XP}d06753 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d06753 to be at Release 3 genomic coordinate 22966055 on chromosome arm 3R. This corresponds to Release 5 coordinate  3R:22976690 . The insertion site of PBac{WH}CG31077f01593 is Release 5 coordinate  3R:22828504 . Consequently, the breakpoints of Df(3R)BSC791 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3R: 22828504;22976690 and the cytological breakpoints predicted from these coordinates are 97D12;97E5. Df(3R)BSC791 failed to complement Df(3R)ED6255 and Df(3R)Exel6206.