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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2009.5.6). Isolation and characterization of Df(3L)BSC801. (Export to RIS) | ||
| FlyBase ID | FBrf0207909 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: Kevin Cook <kercook@XXXXXXXXXXXXXXX>
Date: 6 May 2009 00:43:50 BST To: flybase-cambridgeXXXXXXXXXXXXXXX, sjchristXXXXXXXXXXXXXXX, ruacook@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(3L)BSC801 Isolation and characterization of Df(3L)BSC801 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC801 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Sox21bf06429 and P{XP}CG4914d07633. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}Sox21bf06429/P{XP}CG4914d07633 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC801 from the segment of PBac{WH}Sox21bf06429 to the left of its FRT site and the segment of P{XP}CG4914d07633 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC801 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:14121351;14671335 and the cytological breakpoints predicted from these coordinates are 70D2;70E7. Df(3L)BSC801 failed to complement nufKG02305 and stwlj6C3. -- Kevin Cook, Ph.D Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXXXXXXXXXXXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu |
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| Language of Publication | English | ||
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| Aberrations (1)
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| Alleles (2)
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| Constructs (1)
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| Genes (2)
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| Insertions (3)
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