Isolation and characterization of Df(3L)BSC801
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC801 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Sox21bf06429 and P{XP}CG4914d07633. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}Sox21bf06429/P{XP}CG4914d07633 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC801 from the segment of PBac{WH}Sox21bf06429 to the left of its FRT site and the segment of P{XP}CG4914d07633 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC801 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:14121351 ;14671335 and the cytological breakpoints predicted from these coordinates are 70D2;70E7. Df(3L)BSC801 failed to complement nufKG02305 and stwlj6C3.