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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2009.5.6). Isolation and characterization of Df(3R)BSC809. (Export to RIS) | ||
| FlyBase ID | FBrf0207916 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: Kevin Cook <kercook@XXXXXXXXXXXXXXX>
Date: 6 May 2009 00:44:35 BST To: flybase-cambridgeXXXXXXXXXXXXXXX, sjchristXXXXXXXXXXXXXXX, ruacook@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(3R)BSC809 Isolation and characterization of Df(3R)BSC809 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC809 was isolated as a FLP recombinase-induced recombination event involving P{XP}d10097 and PBac{RB}CG4662e00391. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d10097/PBac{RB}CG4662e00391 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC809 from the segment of P{XP}d10097 to the left of its FRT site and the segment of PBac{RB}CG4662e00391 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(3R)BSC809 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3R:15229135;15672723 and the cytological breakpoints predicted from these coordinates are 92A3;92B3. Df(3R)BSC809 failed to complement bnl00857 and Vha1305113. -- Kevin Cook, Ph.D Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXXXXXXXXXXXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (1)
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| Alleles (2)
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| Constructs (1)
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| Genes (2)
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| Insertions (3)
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