Reference Report

Reference
Citation	Siebert, M., Banovic, D., Goellner, B., Aberle, H. (2009). Drosophila motor axons recognize and follow a Sidestep-labeled substrate pathway to reach their target fields. Genes Dev. 23(9): 1052--1062. (Export to RIS)
FlyBase ID	FBrf0207941
Publication Type	Research paper
PubMed ID	19369411
PubMed Abstract	During development of the Drosophila nervous system, migrating motor axons contact and interact with different cell types before reaching their peripheral muscle fields. The axonal attractant Sidestep (Side) is expressed in most of these intermediate targets. Here, we show that motor axons recognize and follow Side-expressing cell surfaces from the ventral nerve cord to their target region. Contact of motor axons with Side-expressing cells induces the down-regulation of Side. In the absence of Side, the interaction with intermediate targets is lost. Misexpression of Side in side mutants strongly attracts motor axons to ectopic sites. We provide evidence that, on motor axons, Beaten path Ia (Beat) functions as a receptor or part of a receptor complex for Side. In beat mutants, motor axons no longer recognize Side-expressing cell surfaces. Furthermore, Beat interacts with Side both genetically and biochemically. These results suggest that the tracing of Side-labeled cell surfaces by Beat-expressing growth cones is a major principle of motor axon guidance in Drosophila.
DOI	10.1101/gad.520509
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (16)
	Avic\GFP^Fas2-Mue397 beat-Ia³ beat-Ia^C163 beat-Ia^Scer\UAS.cFa Fas2^Mue397 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^btl.PS Scer\GAL4^elav.PLu Scer\GAL4^Fas2-Mz507 Scer\GAL4^how-24B Scer\GAL4^Mef2.PR Scer\GAL4^srp Sh^{Mhc.T:Avic\GFP-S65T,T:Hsap\CD8A} side^C137 side^I1563 side^Scer\UAS.cSa
Constructs (9)
	P{GAL4-btl.S} P{GAL4-elav.L} P{GAL4-Mef2.R} P{Mhc.CD8-GFP-Sh} P{PTT-un1} P{srp-Gal4} P{UAS-beat.F} P{UAS-mCD8::GFP.L} P{UAS-side.S}
Genes (8)
	Avic\GFP beat-Ia Fas2 futsch Mmus\Cd8a Scer\GAL4 Sh side
Insertions (3)
	P{GAL4}Mz507 P{GawB}how^24B P{PTT-un1}Fas2^Mue397