Reference Report

Reference
Citation	Merino, C., Penney, J., González, M., Tsurudome, K., Moujahidine, M., O'Connor, M.B., Verheyen, E.M., Haghighi, P. (2009). Nemo kinase interacts with Mad to coordinate synaptic growth at the Drosophila neuromuscular junction. J. Cell Biol. 185(4): 713--725. (Export to RIS)
FlyBase ID	FBrf0208030
Publication Type	Research paper
PubMed ID	19451277
PubMed Abstract	Bone morphogenic protein (BMP) signaling is essential for the coordinated assembly of the synapse, but we know little about how BMP signaling is modulated in neurons. Our findings indicate that the Nemo (Nmo) kinase modulates BMP signaling in motor neurons. nmo mutants show synaptic structural defects at the Drosophila melanogaster larval neuromuscular junction, and providing Nmo in motor neurons rescues these defects. We show that Nmo and the BMP transcription factor Mad can be coimmunoprecipitated and find a genetic interaction between nmo and Mad mutants. Moreover, we demonstrate that Nmo is required for normal distribution and accumulation of phosphorylated Mad in motor neurons. Finally, our results indicate that Nmo phosphorylation of Mad at its N terminus, distinct from the BMP phosphorylation site, is required for normal function of Mad. Based on our findings, we propose a model in which phosphorylation of Mad by Nmo ensures normal accumulation and distribution of Mad and thereby fine tunes BMP signaling in motor neurons.
DOI	10.1083/jcb.200809127
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (3)
	Df(3L)Exel6279 Df(3L)pbl-NR Df(3L)pbl-X1
Alleles (17)
	Avic\GFP^{E.Scer\UAS.P\T.T:Hsap\mito} Ecol\lacZ^nmo-P1 hiw^EMS Mad⁶ Mad^k00237 Mad^{S25A.Scer\UAS.T:Hsap\MYC} Mad^{Scer\UAS.T:Hsap\MYC} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} nmo^DB24 nmo^{K69M.Scer\UAS.T:Avic\GFP} nmo^P1 nmo^{Scer\UAS.cUa.T:Avic\GFP} nmo^unspecified Scer\GAL4^BG380 Scer\GAL4^G14 Scer\GAL4^Rapgap1-OK6 tkv^{Q199D.Scer\UAS.T:Ivir\HA1}
Constructs (7)
	P{UAS-EGFP-nmo.K69M} P{UAS-GFP-nmo.U} P{UAS-mCD8::GFP.L} P{UAS-Myc-Mad.S25A} P{UAS-Myc-Mad} P{UASp-mito-GFP} P{UAS-tkv^Q199D}
Genes (12)
	Avic\GFP dlg1 Ecol\lacZ Fas2 hiw Mad Mmus\Cd8a nmo Scer\GAL4 T:Avic\GFP T:Hsap\MYC tkv
Insertions (4)
	P{GAL4}BG380 P{GAL4}G14 P{GawB}OK6 P{lacW}nmo^P1
Natural transposons (1)
	P-element