Reference Report

Reference
Citation	Terriente-Félix, A., de Celis, J.F. (2009). Osa, a subunit of the BAP chromatin-remodelling complex, participates in the regulation of gene expression in response to EGFR signalling in the Drosophila wing. Dev. Biol. 329(2): 350--361. (Export to RIS)
FlyBase ID	FBrf0208056
Publication Type	Research paper
PubMed ID	19306864
PubMed Abstract	Gene expression is regulated in part by protein complexes containing ATP-dependent chromatin-remodelling factors of the SWI/SNF family. In Drosophila there is only one SWI/SNF protein, named Brahma, which forms the catalytic subunit of two complexes composed of different proteins. The protein Osa defines the BAP complex, and the proteins Polybromo and Bap170 are only present in the complex named PBAP. In this work we have analysed the functional requirements of Osa during Drosophila wing development, and found that osa is needed for cell growth and survival in the wing imaginal disc, and for the correct patterning of sensory organs, veins and the wing margin. Other members of the BAP complex, such as Snr1, Bap55, Mor and Brm, also share these functions of Osa. We focused on the requirement of Osa during the formation of the wing veins. Genetic interactions between osa alleles and mutations affecting the activity of the EGFR pathway suggest that one aspect of Osa is intimately related to the response to EGFR activity. Thus, loss of osa and EGFR signalling results in similar wing vein phenotypes, and osa alleles enhance the loss of veins caused by reduced EGFR activity. In addition, Osa is required for the expression of several targets of EGFR signalling, such as Delta, rhomboid and argos. We suggest that one role of Osa and Brm in the wing is to establish a chromatin environment in the regulatory regions of EGFR target genes, making them available for both activators and repressors and facilitating transcription in response to EGFR signalling.
DOI	10.1016/j.ydbio.2009.03.010
Related Publication(s)
Personal communication to FlyBase	spalt major-Gal4 lines. de Celis and Basler, 2010.7.11, spalt major-Gal4 lines. [FBrf0211371]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(2R)Px2
Alleles (27)
	Avic\GFP^{S65T.Scer\UAS} Bap170^GD10922 brm² brm^GD4507 bs² Mkp3^Scer\UAS.cRa mor^GD1257 net¹ net^Scer\UAS.cBa osa³⁰⁸ osa^C819 osa^GD1502 osa^Scer\UAS.cCa px⁷² Ras85D^GD12553 Ras85D^N17.Scer\UAS Ras85D^V12.Scer\UAS rho^ve-1 rl^Sem.Scer\UAS Scer\GAL4^C-765 Scer\GAL4^Dll-md23 Scer\GAL4^MD-638 Scer\GAL4^nub-AC-62 Scer\GAL4^salm-459.2 Scer\GAL4^salm.EPv Snr1^GD4140 vn¹
Constructs (14)
	P{GD1257} P{GD1502} P{GD4140} P{GD4507} P{GD10922} P{GD12553} P{salm-GAL4.EPv} P{UAS-GFP.S65T} P{UAS-Mkp3.R} P{UAS-net.B} P{UAS-osa} P{UAS-Ras85D.N17} P{UAS-Ras85D.V12} P{UAS-Sem}
Genes (21)
	aos Avic\GFP Bap55 Bap170 brm bs caup decay Dl kni Mkp3 mor net osa px Ras85D rho rl Scer\GAL4 Snr1 vn
Insertions (6)
	P{GAL4}MD-638 P{GawB}459.2 P{GawB}C-765 P{GawB}Dll^md23 P{GawB}nubbin-AC-62 P{GSV1}osa^C819
Natural transposons (1)
	P-element