Reference Report

Reference
Citation	Ayers, K.L., Rodriguez, R., Gallet, A., Ruel, L., Thérond, P. (2009). Tow (Target of Wingless), a novel repressor of the Hedgehog pathway in Drosophila. Dev. Biol. 329(2): 280--293. (Export to RIS)
FlyBase ID	FBrf0208057
Publication Type	Research paper
PubMed ID	19285058
PubMed Abstract	Hedgehog (Hh) signalling plays a crucial role in the development and patterning of many tissues in both vertebrates and invertebrates. Aberrations in this pathway lead to severe developmental defects and cancer. Hh signal transduction in receiving cells is a well studied phenomenon; however questions still remain concerning the mechanism of repression of the pathway activator Smoothened (Smo) in the absence of Hh. Here we describe a novel repressor of the Hh pathway, Target of Wingless (Tow). Tow represents the Drosophila homolog of a conserved uncharacterised protein family. We show that Tow acts in Hh receiving cells, where its overexpression represses all levels of Hh signalling, and that this repression occurs upstream or at the level of Smo and downstream of the Hh receptor Patched (Ptc). In addition, we find that like Ptc, overexpression of Tow causes an accumulation of lipophorin in the wing disc. We demonstrate that loss of tow enhances different ptc alleles in a similar manner to another pathway repressor, Suppressor of Fused (SuFu), possibly through mediating Ptc dependant lipophorin internalisation. Combined, these results demonstrate that Tow is an important novel regulator of the Hh pathway in the wing imaginal disc, and may shed light on the mechanism of Ptc repression of Smo.
DOI	10.1016/j.ydbio.2009.02.037
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (25)
	cos¹ fu¹ fu^mH63 N^{int.SH.Scer\UAS} ptc¹⁶ ptc^Conf-1 ptc^tuf-1 Scer\GAL4^ap-md544 Scer\GAL4^arm.PS Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E Scer\GAL4^hh-Gal4 Scer\GAL4^ptc-559.1 Scer\GAL4^sd.PU sqh^NIG.3595R Su(fu)^LP tow^{5'UTR.Scer\UAS} tow⁷⁵⁴ tow^A1.Scer\UAS tow^{GH12583.Scer\UAS} tow^UY1146 tow^ΔC.Scer\UAS tow^ΔN.Scer\UAS w^+mC wg^Scer\UAS.cUa
Constructs (11)
	P{GAL4-arm.S} P{Mae-UAS.6.11} P{NIG.3595R} P{sd-GAL4.U} P{UAS-N.intra.SH} P{UAS-tow.5'UTR} P{UAS-tow.A1} P{UAS-tow.GH12583} P{UAS-tow.ΔC} P{UAS-tow.ΔN} P{UAS-wg.U}
Genes (19)
	CG9948 CG10077 CG14820 ci cos dpp Ecol\lacZ en fu N pan ptc Scer\GAL4 smo sqh Su(fu) tow w wg
Insertions (8)
	P{en2.4-GAL4}e16E P{GAL4}hh^Gal4 P{GawB}ap^md544 P{GawB}Bx^MS1096 P{GawB}ptc^559.1 P{Mae-UAS.6.11}tow^UY1146 P{UAS-tow.A1}14 P{UAS-tow.A1}15
Natural transposons (1)
	P-element