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Christensen, S., Cook, K., Cook, K. (2009.6.15). Isolation and characterization of Df(3L)BSC817. 
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From: Kevin Cook <kercook@XXXX>
Date: 15 June 2009  13:13:07  BST
To: FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchrist@XXXX>
Subject: Isolation and characterization of Df(3L)BSC817
Isolation and characterization of Df(3L)BSC817
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC817 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09684 and PBac{RB}e04435. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d09684/PBac{RB}e04435 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC817 from the segment of P{XP}d09684 to the left of its FRT site and the segment of PBac{RB}e04435 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(3L)BSC817 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:11063626 ;11240245 and the cytological breakpoints predicted from these coordinates are 68A4;68B4. Df(3L)BSC817 failed to complement female sterility of l(3)01239j9B4.
Kevin Cook, Ph.D
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
812-856-1213 (office), 812-855-2577 (fax)
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)