Isolation and characterization of Df(1)BSC823
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC823 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f02325 and P{XP}CanBd09902. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 PBac{WH}f02325/ w1118 P{XP}CanBd09902; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC823 from the segment of PBac{WH}f02325 to the left of its FRT site and the segment of P{XP}CanBd09902 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}CanBd09902 to be Release 3 genomic coordinate 5073385 on the X chromosome. This corresponds to 4F5 on the Release 3 and Release 5 genome maps. The insertion site of PBac{WH}f02325 is Release 5 genomic coordinate 5176617--5176617 on the X chromosome. This corresponds to 4F4 on the Release 5 genome map. Consequently, the cytological breakpoints of Df(1)BSC823 are predicted to be 4F4;4F5. It failed to complement snf1.