Reference Report

Reference
Citation	Lin, H.C., Wu, J.T., Tan, B.C., Chien, C.T. (2009). Cul4 and DDB1 regulate Orc2 localization, BrdU incorporation and Dup stability during gene amplification in Drosophila follicle cells. J. Cell Sci. 122(14): 2393--2401. (Export to RIS)
FlyBase ID	FBrf0208137
Publication Type	Research paper
PubMed ID	19531585
PubMed Abstract	In higher eukaryotes, the pre-replication complex (pre-RC) component Cdt1 is the major regulator in licensing control for DNA replication. The Cul4-DDB1-based ubiquitin ligase mediates Cdt1 ubiquitylation for subsequent proteolysis. During the initiation of chorion gene amplification, Double-parked (Dup), the Drosophila ortholog of Cdt1, is restricted to chorion gene foci. We found that Dup accumulated in nuclei in Cul4 mutant follicle cells, and the accumulation was less prominent in DDB1 mutant cells. Loss of Cul4 or DDB1 activity in follicle cells also compromised chorion gene amplification and induced ectopic genomic DNA replication. The focal localization of Orc2, a subunit of the origin recognition complex, is frequently absent in Cul4 mutant follicle cells. Therefore, Cul4 and DDB1 have differential functions during chorion gene amplification.
DOI	10.1242/jcs.042861
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(2R)Exel6056
Alleles (11)
	BacA\p35^Scer\UAS.cHa Cul-4^G1-3 Cul-4^G3-5 Cul-4^G2935 Cul-4^JJ11 Cul-4^KG02900 Cul-4^L2-1 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} pic^5-1 pic^EY01408 Scer\GAL4^tub.PU
Constructs (4)
	P{tub-GAL4.U} P{UAS-Cul-4.Exel} P{UAS-mCD8::GFP.L} P{UAS-p35.H}
Genes (7)
	BacA\p35 Cul-4 dup Mmus\Cd8a Orc2 pic Scer\GAL4
Insertions (2)
	P{EPgy2}pic^EY01408 P{SUPor-P}Cul-4^KG02900
Natural transposons (1)
	P-element