Reference Report

Reference
Citation	Nie, M., Xie, Y., Loo, J.A., Courey, A.J. (2009). Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling, and early pattern formation. PLoS ONE 4(6): e5905. (Export to RIS)
FlyBase ID	FBrf0208144
Publication Type	Research paper
PubMed ID	19529778
PubMed Abstract	SUMO is a protein modifier that is vital for multicellular development. Here we present the first system-wide analysis, combining multiple approaches, to correlate the sumoylated proteome (SUMO-ome) in a multicellular organism with the developmental roles of SUMO. Using mass-spectrometry-based protein identification, we found over 140 largely novel SUMO conjugates in the early Drosophila embryo. Enriched functional groups include proteins involved in Ras signaling, cell cycle, and pattern formation. In support of the functional significance of these findings, sumo germline clone embryos exhibited phenotypes indicative of defects in these same three processes. Our cell culture and immunolocalization studies further substantiate roles for SUMO in Ras signaling and cell cycle regulation. For example, we found that SUMO is required for efficient Ras-mediated MAP kinase activation upstream or at the level of Ras activation. We further found that SUMO is dynamically localized during mitosis to the condensed chromosomes, and later also to the midbody. Polo kinase, a SUMO substrate found in our screen, partially colocalizes with SUMO at both sites. These studies show that SUMO coordinates multiple regulatory processes during oogenesis and early embryogenesis. In addition, our database of sumoylated proteins provides a valuable resource for those studying the roles of SUMO in development.
DOI	10.1371/journal.pone.0005905
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (8)
	lwr¹¹⁸ Scer\GAL4^Bx-MS1096 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} smt3⁰⁴⁴⁹³ smt3^EMS smt3^GD10521 smt3^k01211 smt3^{Scer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6}
Constructs (3)
	P{GD10521} P{matα4-GAL-VP16} P{UAS-smt3.Flag}
Genes (121)
	14-3-3ε 14-3-3ζ Aats-arg Aats-gln Aats-glupro Aos1 asp awd bcd brat cad Caf1 Cas cdc2c CG8142 CG12018 CG12304 CtBP cup dl DNApol-δ Dsor1 eIF-4E emb fs(1)Ya Fs(2)Ket Gnf1 grau gro hb Hel25E His2Av His2B Hrb27C Hsp83 Klp10A lwr mago me31B Meics Mi-2 mod(mdg4) mts mus209 Nacα Nlp osa Pen pho phyl Pitslre polo Pp1-87B Pp2A-29B pum Ras85D retn rl Rpd3 RpL4 RpL7 RpL7A RpL8 RpL9 RpL10Ab RpL11 RpL13 RpL13A RpL14 RpL15 RpL18A RpL22 RpL23 RpL27A RpL28 RpL31 RpL32 RpL36 RpLP0 Rpn2 Rpn3 Rpn7 Rpn11 RpS3 RpS3A RpS4 RpS5a RpS6 RpS7 RpS8 RpS9 RpS10a RpS10b RpS13 RpS14a RpS15Aa RpS16 RpS17 RpS18 RpS19a RpS20 RpS23 RpS25 RpS26 Scer\GAL4 smg smt3 spi sqd Su(var)3-7 Su(var)205 T:Zzzz\FLAG T:Zzzz\His6 Top2 tsr tsu tws Uba2 vas vls αTub67C
Insertions (1)
	P{GawB}Bx^MS1096
Natural transposons (1)
	P-element