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Citation
Li, Y., Minor, N.T., Park, J.K., McKearin, D.M., Maines, J.Z. (2009). Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance.  Proc. Natl. Acad. Sci. U.S.A. 106(23): 9304--9309.
FlyBase ID
FBrf0208161
Publication Type
Research paper
Abstract
The balance between germ-line stem cell (GSC) self-renewal and differentiation in Drosophila ovaries is mediated by the antagonistic relationship between the Nanos (Nos)-Pumilio translational repressor complex, which promotes GSC self-renewal, and expression of Bam, a key differentiation factor. Here, we find that Bam and Nos proteins are expressed in reciprocal patterns in young germ cells. Repression of Nos in Bam-expressing cells depends on sequences in the nos 3'-UTR, suggesting that Nos is regulated by translational repression. Ectopic Bam causes differentiation of GSCs, and this activity depends on the endogenous nos 3'-UTR sequence. Previous evidence showed that Bgcn is an obligate factor for the ability of Bam to drive differentiation, and we now report that Bam forms a complex with Bgcn, a protein related to the RNA-interacting DExH-box polypeptides. Together, these observations suggest that Bam-Bgcn act together to antagonize Nos expression; thus, derepressing cystoblast-promoting factors. These findings emphasize the importance of translational repression in balancing stem cell self-renewal and differentiation.
PubMed ID
PubMed Central ID
PMC2695086 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Aberrations (1)
    Alleles (15)
    Genes (7)
    Physical Interactions (6)
    Polypeptides (2)
    Cell Lines (1)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (7)