Reference Report

Reference
Citation	Patel, P.H., Costa-Mattioli, M., Schulze, K.L., Bellen, H.J. (2009). The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy. J. Cell Biol. 185(7): 1181--1194. (Export to RIS)
FlyBase ID	FBrf0208174
Publication Type	Research paper
PubMed ID	19546244
PubMed Abstract	Hypusination is a unique posttranslational modification by which lysine is transformed into the atypical amino acid hypusine. eIF5A (eukaryotic initiation factor 5A) is the only known protein to contain hypusine. In this study, we describe the identification and characterization of nero, the Drosophila melanogaster deoxyhypusine hydroxylase (DOHH) homologue. nero mutations affect cell and organ size, bromodeoxyuridine incorporation, and autophagy. Knockdown of the hypusination target eIF5A via RNA interference causes phenotypes similar to nero mutations. However, loss of nero appears to cause milder phenotypes than loss of eIF5A. This is partially explained through a potential compensatory mechanism by which nero mutant cells up-regulate eIF5A levels. The failure of eIF5A up-regulation to rescue nero mutant phenotypes suggests that hypusination is required for eIF5A function. Furthermore, expression of enzymatically impaired forms of DOHH fails to rescue nero clones, indicating that hypusination activity is important for nero function. Our data also indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis.
DOI	10.1083/jcb.200904161
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3R)04661
Alleles (23)
	Atg8b^{hs.T:Avic\GFP} CG1910^K48 eIF-5A⁰¹²⁹⁶ eIF-5A^GD8700 eIF-5A^Scer\UAS.cPa Hsap\DOHH^{H56A.Scer\UAS} Hsap\DOHH^{H89A.Scer\UAS} Hsap\DOHH^{H208A.Scer\UAS} Hsap\DOHH^{H240A.Scer\UAS} Hsap\DOHH^Scer\UAS.cPa nero¹ nero² nero^+t1.5 nero^K48-123 nero^s1921 nero^Scer\UAS.cPa Scer\FLP1^ey.PN Scer\GAL4^Act5C.PP Scer\GAL4^Adh.PF Scer\GAL4^dpp.blk1 Scer\GAL4^Eq1 Scer\GAL4^tub.PU Tsc1^Q87X
Constructs (17)
	P{?'lArB} P{ey-FLP.N} P{GAL4-Act5C(-FRT).P} P{GAL4-Adh.Dmul} P{GAL4-dpp.blk1} P{GD8700} P{hs-Atg8b.GFP} P{lacW} P{nero.t.1.5} P{tub-GAL4.U} P{UAS-eIF-5A.P} P{UAS-hDOHH.H56A} P{UAS-hDOHH.H89A} P{UAS-hDOHH.H208A} P{UAS-hDOHH.H240A} P{UAS-hDOHH.WT} P{UAS-nero.P}
Genes (10)
	Atg8b CG1910 dlg1 eIF-5A Hsap\DOHH nero Scer\FLP1 Scer\GAL4 Su(H) Tsc1
Insertions (3)
	P{?'lArB}CG1910^K48 P{GAL4-Hsp70.PB}l(3)Eq1^Eq1 P{lacW}nero^s1921
Natural transposons (1)
	P-element