Reference Report

Reference
Citation	Xu, Y., Lei, Z., Huang, H., Dui, W., Liang, X., Ma, J., Jiao, R. (2009). dRecQ4 is required for DNA synthesis and essential for cell proliferation in Drosophila. PLoS ONE 4(7): e6107. (Export to RIS)
FlyBase ID	FBrf0208182
Publication Type	Research paper
PubMed ID	19572017
PubMed Abstract	The family of RecQ DNA helicases plays an important role in the maintenance of genomic integrity. Mutations in three of the five known RecQ family members in humans, BLM, WRN and RecQ4, lead to disorders that are characterized by predisposition to cancer and premature aging.To address the in vivo functions of Drosophila RecQ4 (dRecQ4), we generated mutant alleles of dRecQ4 using the targeted gene knock-out technique. Our data show that dRecQ4 mutants are homozygous lethal with defects in DNA replication, cell cycle progression and cell proliferation. Two sets of experiments suggest that dRecQ4 also plays a role in DNA double strand break repair. First, mutant animals exhibit sensitivity to gamma irradiation. Second, the efficiency of DsRed reconstitution via single strand annealing repair is significantly reduced in the dRecQ4 mutant animals. Rescue experiments further show that both the N-terminal domain and the helicase domain are essential to dRecQ4 function in vivo. The N-terminal domain is sufficient for the DNA repair function of dRecQ4.Together, our results show that dRecQ4 is an essential gene that plays an important role in not only DNA replication but also DNA repair and cell cycle progression in vivo.
DOI	10.1371/journal.pone.0006107
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Aberrations (1)
	Dp(3;3)RecQ4^+.14
Alleles (16)
	Disc\RFP^{Rr3.Scer\SceI.RS.Act5C} RecQ4^14.3' RecQ4¹⁴ RecQ4^+.5' RecQ4^+t8.6 RecQ4^{FL.Scer\UAS.T:Zzzz\FLAG} RecQ4^Scer\UAS.cXa RecQ4^{TARG.Scer\SceI.RS} RecQ4^{Δ1-807.Scer\UAS.T:Zzzz\FLAG} RecQ4^{Δ1-1207.Scer\UAS.T:Zzzz\FLAG} RecQ4^{Δ868-1207.Scer\UAS.T:Zzzz\FLAG} RecQ4^{Δ868-1579.Scer\UAS.T:Zzzz\FLAG} RecQ4^{Δ1234-1579.Scer\UAS.T:Zzzz\FLAG} Scer\FLP1^ey.PN Scer\GAL4^Act.PU Scer\SCEI^Ubi-p63E.PP
Constructs (13)
	P{Act-GAL4.U} P{Act-RFP.Rr3} P{ey-FLP.N} P{FRT(RecQ4^{TARG.Scer\SceI.RS}.Crei\I-CreI.RS.w⁺)} P{RecQ4-rescue} P{UAS-RecQ4.FL} P{UAS-RecQ4.X} P{UAS-RecQ4.Δ1-807} P{UAS-RecQ4.Δ1-1207} P{UAS-RecQ4.Δ868-1207} P{UAS-RecQ4.Δ868-1579} P{UAS-RecQ4.Δ1234-1579} P{Ubi-p63E-Scer\SCEI}
Genes (6)
	Disc\RFP RecQ4 Scer\FLP1 Scer\GAL4 Scer\SCEI T:Zzzz\FLAG
Insertions (2)
	P{Act-RFP.Rr3}48C P{Ubi-p63E-Scer\SCEI}53D
Natural transposons (1)
	P-element