Reference Report

Reference
Citation	Masuda-Nakagawa, L.M., Gendre, N., O'Kane, C.J., Stocker, R.F. (2009). Localized olfactory representation in mushroom bodies of Drosophila larvae. Proc. Natl. Acad. Sci. U.S.A. 106(25): 10314--10319. (Export to RIS)
FlyBase ID	FBrf0208205
Publication Type	Research paper
PubMed ID	19502424
PubMed Abstract	Odor discrimination in higher brain centers is essential for behavioral responses to odors. One such center is the mushroom body (MB) of insects, which is required for odor discrimination learning. The calyx of the MB receives olfactory input from projection neurons (PNs) that are targets of olfactory sensory neurons (OSNs) in the antennal lobe (AL). In the calyx, olfactory information is transformed from broadly-tuned representations in PNs to sparse representations in MB neurons (Kenyon cells). However, the extent of stereotypy in olfactory representations in the calyx is unknown. Using the anatomically-simple larval olfactory system of Drosophila in which odor ligands for the entire set of 21 OSNs are known, we asked how odor identity is represented in the MB calyx. We first mapped the projections of all larval OSNs in the glomeruli of the AL, and then followed the connections of individual PNs from the AL to different calyx glomeruli. We thus established a comprehensive olfactory map from OSNs to a higher olfactory association center, at a single-cell level. Stimulation of single OSNs evoked strong neuronal activity in 1 to 3 calyx glomeruli, showing that broadening of the strongest PN responses is limited to a few calyx glomeruli. Stereotypic representation of single OSN input in calyx glomeruli provides a mechanism for MB neurons to detect and discriminate olfactory cues.
DOI	10.1073/pnas.0900178106
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (30)
	Avic\GFP^{GCaMP1.3.Scer\UAS.cAa} Avic\GFP^Scer\UAS.cUa Mmus\Cd8a^{Scer\FRT.Scer\UAS.T:Avic\GFP} Mmus\Cd8a^{Scer\UAS.S.T:Avic\GFP} n-syb^{Scer\UAS.cEa.T:Avic\GFP} Orco¹ Orco^T:Hsap\MYC Scer\GAL4^GH146 Scer\GAL4^Or1a.PF Scer\GAL4^Or13a.PF Scer\GAL4^Or22c.PF Scer\GAL4^Or24a.PF Scer\GAL4^Or30a.PF Scer\GAL4^Or33a.PF Scer\GAL4^Or33b.PF Scer\GAL4^Or35a.PF Scer\GAL4^Or42a.PF Scer\GAL4^Or42b.PF Scer\GAL4^Or45a.PF Scer\GAL4^Or45b.PF Scer\GAL4^Or47a.8.239 Scer\GAL4^Or49a.PF Scer\GAL4^Or59a.PF Scer\GAL4^Or63a.PF Scer\GAL4^Or67b.PF Scer\GAL4^Or74a.PF Scer\GAL4^Or82a.PF Scer\GAL4^Or83a.PF Scer\GAL4^Or85c.PF Scer\GAL4^{Orco.2.642.T:Hsim\VP22}
Constructs (28)
	P{Or1a-GAL4.F} P{Or13a-GAL4.F} P{Or22c-GAL4.F} P{Or24a-GAL4.F} P{Or30a-GAL4.F} P{Or33a-GAL4.F} P{Or33b-GAL4.F} P{Or35a-GAL4.F} P{Or42a-GAL4.F} P{Or42b-GAL4.F} P{Or45a-GAL4.F} P{Or45b-GAL4.F} P{Or47a-GAL4.8.239} P{Or49a-GAL4.F} P{Or59a-GAL4.F} P{Or63a-GAL4.F} P{Or67b-GAL4.F} P{Or74a-GAL4.F} P{Or82a-GAL4.F} P{Or83a-GAL4.F} P{Or85c-GAL4.F} P{Orco-GAL4.W} P{Orco-MYC} P{UAS(FRT.CD2.y⁺)CD8-GFP} P{UAS(-FRT)CD8-GFP} P{UAS-GCaMP1.3.A} P{UAS-GFP.U} P{UAS-n-syb-GFP.E}
Genes (28)
	Avic\GFP dlg1 Mmus\Cd8a n-syb Or1a Or13a Or22c Or24a Or30a Or33a Or33b Or35a Or42a Or42b Or45a Or45b Or47a Or49a Or59a Or63a Or67b Or74a Or82a Or83a Or85c Or94b Orco Scer\GAL4
Insertions (1)
	P{GawB}GH146
Transcripts (24)
	Orco^T:Hsap\MYCRA Scer\GAL4^GH146RA Scer\GAL4^Or1a.PFRA Scer\GAL4^Or13a.PFRA Scer\GAL4^Or22c.PFRA Scer\GAL4^Or24a.PFRA Scer\GAL4^Or30a.PFRA Scer\GAL4^Or33a.PFRA Scer\GAL4^Or33b.PFRA Scer\GAL4^Or35a.PFRA Scer\GAL4^Or42a.PFRA Scer\GAL4^Or42b.PFRA Scer\GAL4^Or45a.PFRA Scer\GAL4^Or45b.PFRA Scer\GAL4^Or47a.8.239RA Scer\GAL4^Or49a.PFRA Scer\GAL4^Or59a.PFRA Scer\GAL4^Or63a.PFRA Scer\GAL4^Or67b.PFRA Scer\GAL4^Or74a.PFRA Scer\GAL4^Or82a.PFRA Scer\GAL4^Or83a.PFRA Scer\GAL4^Or85c.PFRA Scer\GAL4^{Orco.2.642.T:Hsim\VP22}RA