Reference Report

Reference
Citation	Lee, W.C., Beebe, K., Sudmeier, L., Micchelli, C.A. (2009). Adenomatous polyposis coli regulates Drosophila intestinal stem cell proliferation. Development 136(13): 2255--2264. (Export to RIS)
FlyBase ID	FBrf0208211
Publication Type	Research paper
PubMed ID	19502486
PubMed Abstract	Adult stem cells define a cellular reserve with the unique capacity to replenish differentiated cells of a tissue throughout an organism's lifetime. Previous analysis has demonstrated that the adult Drosophila midgut is maintained by a population of multipotent intestinal stem cells (ISCs) that resides in epithelial niches. Adenomatous polyposis coli (Apc), a tumor suppressor gene conserved in both invertebrates and vertebrates, is known to play a role in multiple developmental processes in Drosophila. Here, we examine the consequences of eliminating Apc function on adult midgut homeostasis. Our analysis shows that loss of Apc results in the disruption of midgut homeostasis and is associated with hyperplasia and multilayering of the midgut epithelium. A mosaic analysis of marked ISC cell lineages demonstrates that Apc is required specifically in ISCs to regulate proliferation, but is not required for ISC self-renewal or the specification of cell fate within the lineage. Cell autonomous activation of Wnt signaling in the ISC lineage phenocopied Apc loss and Apc mutants were suppressed in an allele-specific manner by abrogating Wnt signaling, suggesting that the effects of Apc are mediated in part by the Wnt pathway. Together, these data underscore the essential requirement of Apc in exerting regulatory control over stem cell activity, as well as the consequences that disrupting this regulation can have on tissue homeostasis.
DOI	10.1242/dev.035196
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (11)
	Apc2³³ Apc2^c9 Apc2^g10 Apc^Q8 arm^{S10.Scer\UAS.T:Hsap\MYC} Avic\GFP^Scer\UAS.cUa Axn^S044230 N^{dsRNA.P.Scer\UAS} pan^ΔN.Scer\UAS Scer\GAL4^esg-NP5130 Scer\GAL4^tub.PU
Constructs (5)
	P{tub-GAL4.U} P{UAS-arm.S10} P{UAS-GFP.U} P{UAS-N.dsRNA.P} P{UAS-pan.dTCFΔN}
Genes (14)
	Apc Apc2 arm Avic\GFP Axn Dl esg His3 N nub pan pros Scer\GAL4 Tk
Insertions (1)
	P{GawB}NP5130