Reference Report

Reference
Citation	Chopra, V.S., Cande, J., Hong, J.W., Levine, M. (2009). Stalled Hox promoters as chromosomal boundaries. Genes Dev. 23(13): 1505--1509. (Export to RIS)
FlyBase ID	FBrf0208218
Publication Type	Research paper
PubMed ID	19515973
PubMed Abstract	Many developmental control genes contain stalled RNA Polymerase II (Pol II) in the early Drosophila embryo, including four of the eight Hox genes. Here, we present evidence that the stalled Hox promoters possess an intrinsic insulator activity. The enhancer-blocking activities of these promoters are dependent on general transcription factors that inhibit Pol II elongation, including components of the DSIF and NELF complexes. The activities of conventional insulators are also impaired in embryos containing reduced levels of DSIF and NELF. Thus, promoter-proximal stalling factors might help promote insulator-promoter interactions. We propose that stalled promoters help organize gene complexes within chromosomal loop domains.
DOI	10.1101/gad.1807309
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (15)
	Ecol\lacZ^{2xPE.Fab-7.IAB5} Ecol\lacZ^{2xPE.Fab-8.iab5} Ecol\lacZ^w.abd-A.iab5 Ecol\lacZ^w.Abd-B.iab5 Ecol\lacZ^w.Antp.iab5 Ecol\lacZ^w.iab5.Abd-B Ecol\lacZ^w.iab5.Ubx Ecol\lacZ^w.iab5.w Ecol\lacZ^w.lab.iab5 Ecol\lacZ^w.Scr.iab5 Ecol\lacZ^w.Ubx.iab5 Nelf-A^KG09483 Nelf-E^c00768 spt4^k05316 Spt5^MGE-3
Constructs (11)
	P{2xPE-Fab-7-IAB5} P{w.2xPE-Fab-8-IAB5-lacZ} P{w.Abd-A-lacZ.iab5} P{w.abd-A-lacZ.iab5} P{w.Antp-lacZ.iab5} P{w.iab.Adb-B-lacZ} P{w.iab.Ubx-lacZ} P{w.iab.w-lacZ} P{w.lab-lacZ.iab5} P{w.Scr-lacZ.iab5} P{w.Ubx-lacZ.iab5}
Genes (22)
	abd-A Abd-B Antp C15 Ecol\lacZ Fab-7 Fab-8 Hsp70Aa Hsp70Ab Hsp70Ba Hsp70Bb Hsp70Bbb Hsp70Bc lab lbl Nelf-A Nelf-E Scr spt4 Spt5 tin Ubx
Insertions (3)
	P{lacW}spt4^k05316 P{SUPor-P}Nelf-A^KG09483 PBac{PB}Nelf-E^c00768
Natural transposons (1)
	P-element