Reference Report

Reference
Citation	Dienstbier, M., Boehl, F., Li, X., Bullock, S.L. (2009). Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor. Genes Dev. 23(13): 1546--1558. (Export to RIS)
FlyBase ID	FBrf0208220
Publication Type	Research paper
PubMed ID	19515976
PubMed Abstract	Cytoplasmic sorting of mRNAs by microtubule-based transport is widespread, yet very little is known at the molecular level about how specific transcripts are linked to motor complexes. In Drosophila, minus-end-directed transport of developmentally important transcripts by the dynein motor is mediated by seemingly divergent mRNA elements. Here we provide evidence that direct recognition of these mRNA localization signals is mediated by the Egalitarian (Egl) protein. Egl and the dynein cofactor Bicaudal-D (BicD) are the only proteins from embryonic extracts that are abundantly and specifically enriched on RNA localization signals from transcripts of gurken, hairy, K10, and the I factor retrotransposon. In vitro assays show that, despite lacking a canonical RNA-binding motif, Egl directly recognizes active localization elements. We also reveal a physical interaction between Egl and a conserved domain for cargo recruitment in BicD and present data suggesting that Egl participates selectively in BicD-mediated transport of mRNA in vivo. Our work leads to the first working model for a complete connection between minus-end-directed mRNA localization signals and microtubules and reveals molecular strategies that are likely to be of general relevance for cargo transport by dynein.
DOI	10.1101/gad.531009
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (14)
	BicD^{K730M.tub.T:Avic\GFP-EGFP} BicD^r5 BicD^{Scer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6} BicD^{tub.T:Avic\GFP-EGFP} BicD^{tub.T:MS2\MCP} BicD^{ΔC.tub.T:MS2\MCP} egl¹ egl⁴ egl^{Scer\UAS.P\T.cBa} egl^{tub.T:Avic\GFP-EGFP} Rab6^eAM4 Rab6^ex23D Scer\GAL4^{nos.UTR.T:Hsim\VP16} T:Avic\GFP^EGFP
Constructs (8)
	P{GAL4::VP16-nos.UTR} P{tub-BicD.CP} P{tub-BicD.EGFP} P{tub-BicD.K730M.EGFP} P{tub-BicD.ΔC.CP} P{tub-egl.EGFP} P{UASp-BicD.FLAG.His6} P{UASp-egl.B}
Genes (13)
	BicD egl elav fs(1)K10 ftz grk h lost Rab6 run Scer\GAL4 T:MS2\MCP yps
Natural transposons (2)
	I-element P-element