Reference Report

Reference
Citation	Venkiteswaran, G., Hasan, G. (2009). Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight. Proc. Natl. Acad. Sci. U.S.A. 106(25): 10326--10331. (Export to RIS)
FlyBase ID	FBrf0208231
Publication Type	Research paper
PubMed ID	19515818
PubMed Abstract	Neuronal Ca(2+) signals can affect excitability and neural circuit formation. Ca(2+) signals are modified by Ca(2+) flux from intracellular stores as well as the extracellular milieu. However, the contribution of intracellular Ca(2+) stores and their release to neuronal processes is poorly understood. Here, we show by neuron-specific siRNA depletion that activity of the recently identified store-operated channel encoded by dOrai and the endoplasmic reticulum Ca(2+) store sensor encoded by dSTIM are necessary for normal flight and associated patterns of rhythmic firing of the flight motoneurons of Drosophila melanogaster. Also, dOrai overexpression in flightless mutants for the Drosophila inositol 1,4,5-trisphosphate receptor (InsP(3)R) can partially compensate for their loss of flight. Ca(2+) measurements show that Orai gain-of-function contributes to the quanta of Ca(2+)-release through mutant InsP(3)Rs and elevates store-operated Ca(2+) entry in Drosophila neurons. Our data show that replenishment of intracellular store Ca(2+) in neurons is required for Drosophila flight.
DOI	10.1073/pnas.0902982106
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (14)
	Ca-P60A^Kum170 Itp-r83A^ka1091 Itp-r83A^ug3 mAcR-60C^Scer\UAS.cVa olf186-F^EY01467 olf186-F^GD3554 olf186-F^k11505 olf186-F^Scer\UAS.cZa Scer\GAL4^Ddc.PL Scer\GAL4^elav-C155 Scer\GAL4^hs.PB Scer\GAL4^Ilp2.PR Scer\GAL4^VGlut-OK371 Stim^GD16187
Constructs (7)
	P{Ddc-GAL4.L} P{GAL4-Hsp70.PB} P{GD3554} P{GD16187} P{Ilp2-GAL4.R} P{UAS-mAChR.V} P{UAS-Orai.Z}
Genes (7)
	Ca-P60A Itp-r83A mAcR-60C Nmdar1 olf186-F Scer\GAL4 Stim
Insertions (2)
	P{GawB}elav^C155 P{GawB}VGlut^OK371
Natural transposons (1)
	P-element