Reference Report

Reference
Citation	Rajan, A., Tien, A.C., Haueter, C.M., Schulze, K.L., Bellen, H.J. (2009). The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors. Nat. Cell Biol. 11(7): 815--824. (Export to RIS)
FlyBase ID	FBrf0208249
Publication Type	Research paper
PubMed ID	19543274
PubMed Abstract	Cell fate decisions mediated by the Notch signalling pathway require direct cell-cell contact between adjacent cells. In Drosophila melanogaster, an external sensory organ (ESO) develops from a single sensory organ precursor (SOP) and its fate specification is governed by differential Notch activation. Here we show that mutations in actin-related protein-3 (Arp3) compromise Notch signalling, leading to a fate transformation of the ESO. Our data reveal that during ESO fate specification, most endocytosed vesicles containing the ligand Delta traffic to a prominent apical actin-rich structure (ARS) formed in the SOP daughter cells. Using immunohistochemistry and transmission electron microscopy (TEM) analyses, we show that the ARS contains numerous microvilli on the apical surface of SOP progeny. In Arp2/3 and WASp mutants, the surface area of the ARS is substantially reduced and there are significantly fewer microvilli. More importantly, trafficking of Delta-positive vesicles from the basal area to the apical portion of the ARS is severely compromised. Our data indicate that WASp-dependent Arp2/3 actin polymerization is crucial for apical presentation of Delta, providing a mechanistic link between actin polymerization and Notch signalling.
DOI	10.1038/ncb1888
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (7)
	Df(3L)66C-G28 Df(3L)Exel7591 Df(3L)Exel7593 Df(3L)Exel7930 Df(3L)Exel8065 Df(3L)pbl-X1 Df(3L)ZP1
Alleles (17)
	AP-2α^ear4 Arp3^83F Arp3^515FC Arp3^1066PC Arp3^EP3640 Arp3^{Scer\UAS.P\T.T:Avic\GFP} Arpc1^Q25st Dl^Scer\UAS.cUa N^ECN.Scer\UAS neur¹¹ numb² Rnor\CD2^{Scer\UAS.T:Arus\HRP,T:SS-wg} Scer\FLP1^Ubx.hs Scer\GAL4^{neur-GAL4-A101} Scer\GAL4^sca-109-68 Scer\GAL4^αTub84B.PL WASp³
Constructs (6)
	P{tubP-GAL4} P{UAS-CD2::HRP} P{UAS-Dl.U} P{UAS-N.ECN} P{UASp-Arp3.GFP} P{Ubx-FLP}
Genes (21)
	AP-2α Arp3 Arpc1 ct Dl dlg1 elav Fas3 mwh Myo61F N neur numb peb Rnor\CD2 Scer\FLP1 Scer\GAL4 shg shi sns WASp
Insertions (3)
	P{EP}Arp3^EP3640 P{GawB}neur^GAL4-A101 P{GawB}sca^109-68