Reference Report

Reference
Citation	Massarwa, R., Schejter, E.D., Shilo, B.Z. (2009). Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. Dev. Cell 16(6): 877--888. (Export to RIS)
FlyBase ID	FBrf0208270
Publication Type	Research paper
PubMed ID	19531358
PubMed Abstract	Apical localization of filamentous actin (F-actin) is a common feature of epithelial tubes in multicellular organisms. However, its origins and function are not known. We demonstrate that the Diaphanous (Dia)/Formin actin-nucleating factor is required for generation of apical F-actin in diverse types of epithelial tubes in the Drosophila embryo. Dia itself is apically localized both at the RNA and protein levels, and apical localization of its activators, including Rho1 and two guanine exchange factor proteins (Rho-GEFs), contributes to its activity. In the absence of apical actin polymerization, apical-basal polarity and microtubule organization of tubular epithelial cells remain intact; however, secretion through the apical surface to the lumen of tubular organs is blocked. Apical secretion also requires the Myosin V (MyoV) motor, implying that secretory vesicles are targeted to the apical membrane by MyoV-based transport, along polarized actin filaments nucleated by Dia. This mechanism allows efficient utilization of the entire apical membrane for secretion.
DOI	10.1016/j.devcel.2009.04.010
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (28)
	Arp3^GD12273 Arpc1^GD14085 Avic\GFP^Scer\UAS.cUa dia⁵ dia^{CA.Scer\UAS.T:Ivir\HA1} dia^GD9442 dia^NIG.1768R dia^{Scer\UAS.T:Avic\GFP-EGFP} didum^{CT.Scer\UAS.T:Avic\GFP} didum^GD1848 didum^Q1052st dor^GD10124 Khc::nod^{Scer\UAS.T:Ecol\lacZ} Moe^{Scer\UAS.T:Avic\GFP} Pkn^{G58A.Scer\UAS.T:Avic\GFP-EGFP} Rab11^{SN.cGa.Scer\UAS} Rho1^GD4726 Rho1^N19.Scer\UAS Rho1^V14.Scer\UAS RhoGEF2⁰⁴²⁹¹ RhoGEF2^NIG.9635R RhoGEF64C²⁹ RhoGEF64C^GD16275 Sar1^11-3-63 Sar1²⁸ Scer\GAL4^btl.PS Scer\GAL4^drm.7.1 Scer\GAL4^Mef2.PR
Constructs (22)
	P{drm-GAL4.7.1} P{GAL4-btl.S} P{GAL4-Mef2.R} P{GD1848} P{GD4726} P{GD9442} P{GD10124} P{GD12273} P{GD14085} P{GD16275} P{NIG.1768R} P{NIG.9635R} P{UAS-dia.CA} P{UAS-didum.CT-GFP} P{UAS-GFP.U} P{UAS-Khc::nod::lacZ} P{UAS-Moe::GFP} P{UASp-dia.EGFP} P{UAS-Pkn.G58A.eGFP} P{UAS-Rab11.SN.G} P{UAS-Rho1.N19} P{UAS-Rho1.V14}
Genes (25)
	arm Arp3 Arpc1 Avic\GFP crb dia didum dlg1 dor Fas2 Fas3 Khc::nod Moe pio Pkn Rab11 Rho1 RhoGEF2 RhoGEF64C Sar1 Scer\GAL4 serp shg trh verm
Natural transposons (1)
	P-element