Isolation and characterization of Df(3R)BSC849
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC849 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG10560f00512 and P{XP}d03120. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG10560f00512/P{XP}d03120 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC849 from the segment of PBac{WH}CG10560f00512 to the left of its FRT site and the segment of P{XP}d03120 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC849 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:21146224--21146227 ;21341511
and the cytological breakpoints predicted from these coordinates are 96D1;96E2. Df(3R)BSC849 failed to complement Df(3R)BSC522 and Df(3R)Exel6202.