Reference Report

Reference
Citation	Evans, C.J., Olson, J.M., Ngo, K.T., Kim, E., Lee, N.E., Kuoy, E., Patananan, A.N., Sitz, D., Tran, P., Do, M.T., Yackle, K., Cespedes, A., Hartenstein, V., Call, G.B., Banerjee, U. (2009). G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila. Nat. Methods 6(8): 603--605. (Export to RIS)
FlyBase ID	FBrf0208355
Publication Type	Research paper
External Crossreferences
PubMed ID	19633663
PubMed Abstract	We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
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Text of Personal Communication
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Supplementary material	Online methods. [FBrf0208737]
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Nat. Methods
Title	Nature Methods
Editors
Publication Year	2004-
ISBN/ISSN	1548-7091 1548-7105
Data from Reference
Alleles (15)
	Avic\GFP^{Scer\FRT.STOP.Ubi-p63E.T:nls5} Avic\GFP^{Ubi-p63E.T:nls5} Disc\RFP^{DsRedT4.Scer\UAS.T:nls5} Scer\FLP1^Scer\UAS.cDa Scer\GAL4^{CG33090-NP0189} Scer\GAL4^Cg.PA Scer\GAL4^dpp.blk1 Scer\GAL4^Ef2b-NP0829 Scer\GAL4^ey-OK107 Scer\GAL4^HCH.Hand Scer\GAL4^He.PZ Scer\GAL4^hh-Gal4 Scer\GAL4^NP0114 Scer\GAL4^ptc-559.1 Scer\GAL4^upd-E132
Constructs (8)
	P{Cg-GAL4.A} P{GAL4-dpp.blk1} P{HCH-GAL4} P{He-GAL4.Z} P{UAS-FLP1.D} P{UAS-RedStinger} P{Ubi-p63E(FRT.STOP)Stinger} P{Ubi-p63E(-FRT)Stinger}
Genes (4)
	Avic\GFP Disc\RFP Scer\FLP1 Scer\GAL4
Insertions (7)
	P{GAL4}hh^Gal4 P{GawB}CG33090^NP0189 P{GawB}E132 P{GawB}Ef2b^NP0829 P{GawB}ey^OK107 P{GawB}NP0114 P{GawB}ptc^559.1