Reference Report

Reference
Citation	Hödl, M., Basler, K. (2009). Transcription in the absence of histone H3.3. Curr. Biol. 19(14): 1221--1226. (Export to RIS)
FlyBase ID	FBrf0208395
Publication Type	Research paper
PubMed ID	19523831
PubMed Abstract	Di- and trimethylation of histone H3 lysine 4 (H3K4me2 and H3K4me3) are hallmarks of chromatin at active genes. The major fraction of K4-methylated histone H3 is the variant H3 (termed H3.3 in Drosophila), which replaces canonical H3 (H3.2) in transcribed genes. Here, we genetically address the in vivo significance of such K4 methylation by replacing wild-type H3.3 with a mutant form (H3.3K4A) that cannot be methylated. We monitored the transcription that occurs in response to multiple well-described signaling pathways. Surprisingly, the transcriptional outputs of these pathways remain intact in H3.3K4A mutant cells. Even the complete absence of both H3.3 genes does not noticeably affect viability or function of cells: double mutant animals are viable but sterile. Fertility can be rescued by K4-containing versions of H3.3, but not with mutant H3.3 (H3.3K4A) or with canonical H3.2. Together, these data suggest that in Drosophila, presence of H3.3K4me in the chromatin of active genes is dispensable for successful transcription in most cells and only plays an important role in reproductive tissues.
DOI	10.1016/j.cub.2009.05.048
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (1)
	Df(1)His3.3B^KO
Alleles (16)
	Avic\GFP^E.Ubi His3.3A^KO His3.3B^cHa His3.3B^{H3.2.T:Ivir\HA1} His3.3B^{K4A.T:Ivir\HA1} His3.3B^{K9A.T:Ivir\HA1} His3.3B^KO His3.3B^Scer\FRT.cHa His3.3B^{Scer\FRT.H3.2.T:Ivir\HA1} His3.3B^{Scer\FRT.K4A.T:Ivir\HA1} His3.3B^{Scer\FRT.K9A.T:Ivir\HA1} His3.3B^{Scer\FRT.T:Ivir\HA1} His3.3B^T:Ivir\HA1 His3.3B^T:Zzzz\FLAG Scer\GAL4^His3.3B.PH Scer\GAL4^{Scer\FRT.His3.3B}
Constructs (14)
	P{?'RS5} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)GAL4} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.H3.2.HA} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K4A.HA} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.K9A.HA} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT.HA} P{His3.3B(FRT.His3.3B.WT.FLAG.ubiGFP)His3.3B.WT} P{His3.3B(-FRT)GAL4} P{His3.3B(-FRT)His3.3B.H3.2.HA} P{His3.3B(-FRT)His3.3B.K4A.HA} P{His3.3B(-FRT)His3.3B.K9A.HA} P{His3.3B(-FRT)His3.3B.WT.HA} P{His3.3B(-FRT)His3.3B.WT} P{RS5}
Genes (15)
	Avic\GFP CG11042 CG14036 ct dalao His3.3A His3.3B Ost48 ptc Rrp40 salm Scer\GAL4 sens T:Ivir\HA1 T:Zzzz\FLAG
Insertions (4)
	P{?'RS5}His3.3A^KO P{RS5}His3.3A^5-HA-1043 P{XP}His3.3B^d04051 PBac{RB}e02425
Natural transposons (1)
	P-element