Reference Report

Reference
Citation	Feng, Y., Irvine, K.D. (2009). Processing and phosphorylation of the Fat receptor. Proc. Natl. Acad. Sci. U.S.A. 106(29): 11989--11994. (Export to RIS)
FlyBase ID	FBrf0208432
Publication Type	Research paper
PubMed ID	19574458
PubMed Abstract	The Drosophila tumor suppressors fat and discs overgrown (dco) function within an intercellular signaling pathway that controls growth and polarity. fat encodes a transmembrane receptor, but post-translational regulation of Fat has not been described. We show here that Fat is subject to a constitutive proteolytic processing, such that most or all cell surface Fat comprises a heterodimer of stably associated N- and C-terminal fragments. The cytoplasmic domain of Fat is phosphorylated, and this phosphorylation is promoted by the Fat ligand Dachsous. dco encodes a kinase that influences Fat signaling, and Dco is able to promote the phosphorylation of the Fat intracellular domain in cultured cells and in vivo. Evaluation of dco mutants indicates that they affect Fat's influence on growth and gene expression but not its influence on planar cell polarity. Our observations identify processing and phosphorylation as post-translational modifications of Fat, correlate the phosphorylation of Fat with its activation by Dachsous in the Fat-Warts pathway, and enhance our understanding of the requirement for Dco in Fat signaling.
DOI	10.1073/pnas.0811540106
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	Df(2L)ED94
Alleles (18)
	Avic\GFP^Scer\UAS.cUa CkIα^{Scer\UAS.T:Zzzz\FLAG} d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} dco^{3.Scer\UAS.T:SV5\V5,T:Zzzz\His6} dco³ dco^KD.Scer\UAS ds^36D ds^Scer\UAS.cTa fj^d1 fj^Scer\UAS.cZa ft⁸ ft^G-rv ft^{Scer\UAS.T:Zzzz\FLAG,T:SV5\V5,T:Zzzz\His6} ft^T:SV5\V5 ft^unspecified Scer\GAL4^en.PU Scer\GAL4^tub.PU wts^{Scer\UAS.T:Hsap\MYC}
Constructs (12)
	P{en-GAL4.U} P{ft-V5.F} P{tub-GAL4.U} P{UAS-CkIα.FLAG} P{UAS-d.V5} P{UAS-dco.3} P{UAS-dco.KD} P{UAS-ds.T} P{UAS-fj} P{UAS-ft.FVH} P{UAS-GFP.U} P{UAS-wts.MYC}
Genes (14)
	Avic\GFP CkIα d dco ds fj ft per Scer\GAL4 T:SV5\V5 T:Zzzz\FLAG T:Zzzz\His6 wg wts
Natural transposons (1)
	P-element